薏苡仁油融合指纹图谱研究  

Research on fusion-fingerprint of Coicis Semen oil

作　　者：崔媛[1] 王小明[1] 杨勇[2] 容蓉[1] 巩丽丽[3] 蒋海强[3] 韩冰冰[2] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学基础医学院,山东济南250355 [3]山东中医药大学实验中心,山东济南250355

出　　处：《中草药》2014年第12期1698-1701,共4页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础研究发展计划"973"课题(2013CB531803)

摘　　要：目的建立薏苡仁油的融合指纹图谱。方法薏苡仁油经气相色谱-质谱联用(GC-MS)和高效液相色谱-蒸发光散射(HPLC-ELSD)检测,2种分析方法所得数据进行峰面积标准化处理,运用中药色谱指纹图谱相似度评价软件进行指纹图谱融合。结果薏苡仁融合指纹谱获得21个共有峰,GC-MS技术鉴定出13种脂肪酸酯类成分;HPLC-ELSD法检测部分得到8个共有峰。结论融合指纹图谱的整体性好,信息量大,结合质谱技术部分消除了单一测定方法指纹图谱的模糊性,为中药质量控制研究提供了一定的参考。Objective To establish the fusion-fingerprint of Coicis Semen oil. Methods The Coicis Semen oil was analyzed by GC-MS and HPLC-ELSD, and the fusion-fingerprint was originated from the Computer Aided Similarity Evaluation software based on the normalized data acquired by the above two methods. Results Twenty-one common peaks existed in the fusion-fingerprint of which there were 13 kinds of aliphatic esters detected by GC-MS and 8 kinds of triglyceride ingredients detected by HPLC-ELSD. Conclusion The fusion-fingerprint established on GC-MS combined with HPLC-ELSD could provide integrity and more information for the quality control of Chinese materia medica.

关 键 词：薏苡仁油 融合指纹图谱 气相色谱-质谱联用 高效液相色谱-蒸发光散射检测器 脂肪酸酯 

分 类 号：R286.02[医药卫生—中药学]