酯化型表没食子儿茶素没食子酸酯对乳腺癌细胞凋亡的影响  

Effect of esterified epigallocatechin gallate on apoptosis of breast cancer cells

作　　者：昌丽静 王晶[2,3] 王鹏程[2,3] 王宣军[1,2,3] 盛军[2,3] 

机构地区：[1]云南农业大学龙润普洱茶学院,云南昆明650201 [2]云南农业大学普洱茶学教育部重点实验室,云南昆明650201 [3]云南省茶深加工工程技术研究中心,云南昆明650201

出　　处：《中国生物制品学杂志》2014年第6期764-769,共6页Chinese Journal of Biologicals

基　　金：云南省科技创新强省计划(2009AB021);云南省自然科学基金面上项目(2012FB151)

摘　　要：目的探讨酯化型表没食子儿茶素没食子酸酯(epigallocatechin gallate,EGCG)对人乳腺癌细胞株MCF-7(Myc依赖性)和MDA-MB-231(Myc非依赖性)凋亡的影响。方法采用脂肪酶催化法对EGCG进行酯化修饰,2次硅胶柱层析纯化后,通过高效液相色谱-质谱(HPLC-MS)进行鉴定,并分析纯化的酯化型EGCG的稳定性。流式细胞术检测纯化的酯化型EGCG对MCF-7和MDA-MB-231细胞凋亡的影响。结果经酶法修饰并纯化后获得了EGCG单棕榈酸酯(EGCG-C16),HPLC中不同保留时间的酯化产物的相对分子质量一致,均为696;EGCG在普通培养基里产生的H2O2量远高于其在酸性培养基里及EGCG-C16在普通培养基里产生的H2O2量(P<0.000 1),表明EGCG-C16的稳定性较EGCG好;EGCG-C16对MCF-7细胞具有明确的促进凋亡效应,而对MDA-MB-231细胞则无此效应。结论酯化型EGCG对Myc依赖性乳腺癌细胞具有特异性的促进凋亡效应,为进一步深入研究EGCG的抗肿瘤效应奠定了基础。Objective To investigate the effect of esterified epigallocatechin gallate （EGCG） on apoptosis of Myc-dependent MCF-7 and Myc-independent MDA-MB-231 strains of human breast cancer cells. Methods EGCG was esterified by lipase catalysis, purified by silica gel column chromatography for 2 times, identified by HPLC-MS, and analyzed for stability. The effect of esterified EGCG on apoptosis of MCF-7 and MDA-MB-231 cells were evaluated by flow cytometry. Results EGCG-C16 was obtained after modification and purification. All the relative molecular masses of esterified products at various retention time points in HPLC were 696. The hydrogen peroxide level produced by EGCG in common medium was significantly higher than that by EGCG in acidic medium and that by EGCG-C16 in common medium （P 〈 0. 000 1 ）, indicating that the stability of EGCG-C16 was higher than that of EGCG. EGCG-C16 showed significantly promoting effect on the apoptosis of MCF-7 cells, while showed no the effect on MDA-MB-231 cells. Conclusion Esterified EGCG promoted the apoptosis of Myc-dependent breast cancer cells specifically, which laid a foundation of further study on the anti-tumor effect of EGCG.

关 键 词：表没食子儿茶素没食子酸酯 酯化 乳腺癌 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤] Q255[医药卫生—临床医学]