大鼠肝癌变过程中几种癌基因的原位表达及c-Ha-ras1点突变的研究  

Expression of some oncogenes and point mutation of c-Ha-ras1 during hepatocarcinogenesis in rats

作　　者：张新立[1] 史景泉[1] 卞修武[1] 

机构地区：[1]第三军医大学附属西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2001年第3期308-311,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 !(3 92 70 3 0 3 )

摘　　要：目的　研究大鼠肝癌变过程中几种癌基因的表达和c Ha ras 1点突变。方法　通过DEN诱发癌变启动模型和肝癌模型 ,应用免疫组化、原位杂交和MDT PCR SSCP分别观测癌基因的表达和c Ha ras1点突变。结果　c Ha ras和c myc基因的过表达参与了肝癌变的全过程 ,其中c Ha ras的过表达与癌前嗜碱性肝细胞灶、c myc基因的过表达与卵圆细胞增殖关系密切。IGF Ⅱ基因的过表达对癌前肝细胞增生灶 /结节向肝细胞癌的演进起重要作用。fms基因过表达仅与个别大鼠肝细胞癌有关。结论　大鼠肝癌变过程与c Ha ras、c myc、IGF Ⅱ和fms基因的过表达及c Ha ras1点突变有关 。Objective To study the expressions of oncogenes c Ha ras, c ki ras, pan ras and c myc and point mutation of c Ha ras1 during hepatocarcinogenesis in rats. Methods Immunohistochemistry, in situ hybridization and microdissection of tissue (MDT) PCR SSCP were used to detect the oncogene expressions and point mutation of c Ha ras1 in both Solt Farber model and DEN induced liver cancer model. Results The overexpression of c Ha ras was closely associated with the formation and proliferation of the precancerous basophilic hepatocyte foci, while that of c myc with the growth of the oval cell foci. The abnormalities of IGF Ⅱ played an important role in the evolution of precancerous foci/nodules towards hepatocellular carcinoma (HCC). The overexpression of fms was only associated with HCC of some rats. Conclusion Hepatocarcinogenesis in rats was related with the overexpression of c Ha ras, c myc, IGF Ⅱand fms and the point mutation of c Ha ras1, and overexpression of these oncogenes was associated with morphological evolution.

关 键 词：肝癌变 癌基因 单链构像多态件 c-Ha-ras1 基因点突变 原位表达 

分 类 号：R735.7[医药卫生—肿瘤]