肝豆状核变性基因在皮肤成纤维细胞株转染后的表达  

Detection of Wilson disease gene expression in transfected Menkes disease patient's fibroblast.

作　　者：闫振文[1] 梁秀龄[2] 杨春水[1] 侯国庆[1] 徐评议[2] 陈嵘[2] 黄智恒[1] 黄帆[2] 

机构地区：[1]中山医科大学附属第一医院神经科研究生,广州510080 [2]中山医科大学附属第一医院神经科

出　　处：《中国神经精神疾病杂志》2001年第2期83-85,共3页Chinese Journal of Nervous and Mental Diseases

基　　金：卫生部临床学科重大项目 !(编号 :370 91);中山医科大学"2 11工程"重点建设项目;广东省自然科学基金 !(编号 :96 0 12 8);广东

摘　　要：目的　利用逆转录聚合酶链反应 (RT PCR)法在核酸水平检测并证实肝豆状核变性 (Wilson病 ,WD)基因可以在Me32aT2 2细胞株中得到有效表达 ,为以后更深入的研究奠定基础。方法　采用脂质体转染法将WDcDNA重组真核表达载体pRc/CMV WD导入无血清的Me32aT2 2 /2L细胞株 ,RT PCR在核酸水平检测WD基因在Me32aT2 2 /2L细胞株的表达。结果　在转染的Me32aT2 2 /2L细胞株中可检测到WDcDNA表达 ,而在未转染的Me32aT2 2 /2L细胞中RT PCR检测结果呈阴性。结论　WD基因转染至Me32aT2 2Objective Determination of Wilson disease gene mRNA expression in human fibroblast cell strain (Me32aT22/2L) by reverse transcription polymerase chain reaction (RT PCR). Methods Using lipofection reagent, the plasmid vector carrying the Wilson disease gene (pRc/CMV WD) was transferred into Me32aT22/2L cultured in serum free complement medium. RT PCR was used to determine WD mRNA expression in Me32aT22/2L. Results Wilson disease gene expression was detected in Me32aT22/2L, while no specific signals were detected in untransfected fibroblast. Conclusions It demonstrated that Me32aT22/2L strain could express the Wilson disease gene, suggesting that Wilson disease gene transfer might develop a new approach to study Wilson disease.

关 键 词：肝豆状核变性 基因表达 逆转录聚合酶链反应 成纤维细胞株 

分 类 号：R742.4[医药卫生—神经病学与精神病学]