人肝癌特异性EB病毒载体-p^(EBAF)介导hGM-CSF基因的转移及表达  

hGM-CSF GENE TRANSFER MEDIATED BY HUMAN HEPATOCELLULAR CARCINOMA (HCC) TISSUE SPECIFIC EBV-BASED VECTOR P^(EBAF) AND ITS TISSUE-SPECIFIC EXPRESSION

作　　者：李玉富[1] 张俊萍[1] 孙声桃[2] 李继昌[2] 李福胜[1] 张智清[1] 

机构地区：[1]河南省肿瘤医院内科,郑州450003 [2]河南医科大学,郑州450052

出　　处：《中国现代医学杂志》2001年第3期17-19,共3页China Journal of Modern Medicine

基　　金："863"高科技计划资助项目! (863 -10 2 -11-0 9)

摘　　要：目的 :探索人粒细胞 -巨噬细胞集落刺激因子 (hGM -CSF)基因在肝癌细胞中特异性表达的新途径。方法 :构建 pEBAF/hGM -CSF基因克隆 ,用脂质体转染法将它分别导入分泌甲胎蛋白 (AFP)的肝癌细胞株HepG2和不分泌APF的细胞株SMMC772 1,构建两转基因的细胞克隆HepG2 /hGM -CSF、SMMC772 1/hGM-CSF ,用MTT比色法测定细胞上清中hGM -CSF活性。结果 :HepG2 /hGM -CSF细胞上清hGM -CSF活性平均为 46 .5ng/ml/10 6/2 4h ,SMMC772 1细胞上清中无明显hGM -CSF活性。结论Objective:To investigate a new way of tissue-specific expression of human granulocyte-macrophage colony stimulating factor (hGM-CSF) gene in HCC.Methods:Construct p EBAF/hGF-CSF,transferred into HCC cell line HepG2 (secreting AFP) and SMMC7721 (not secreting AFP) by lipofectin,two positive cell lines HepG2/hGM-CSF and SMMC7721/hGM-CSF.Results:By MTT assay the cytokine biological activity revealed the HepG2/hGM-CSF expressed hGM-CSF for 46.5ng/ml/106/24hr,but SMMC7721 has no expression of hGM-CSF were established.Conclusions:hGM-CSF gene mediated by p EBAF has the ablility of tissue-specific expression in HCC cells.

关 键 词：EB病毒表达载体 人粒细胞-巨噬细胞集落刺激因子 基因治疗 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]