脂质体介导的基因转染效率与肺癌细胞的增殖特性的关系  

The Relationship between the Efficiency of Cationic Liposome-Mediated Transfection and the Proliferation Properties of Lung Cancer Cells

作　　者：李芳[1] 伍新尧[2] 徐平[1] 区敬华[1] 童大跃[1] 

机构地区：[1]中山医科大学生物化学教研室,广州510089 [2]中山医科大学法医学系

出　　处：《中国肿瘤生物治疗杂志》2001年第1期41-44,共4页Chinese Journal of Cancer Biotherapy

基　　金：在读博士研究生项目资助! (3 3 12 2 6)

摘　　要：目的 :探讨阳离子脂质体介导的基因转染效率与肺癌细胞增殖特性的关系。方法 :采用pCMVβ报告基因质粒及自杀基因E .colicd的真核表达载体评价脂质体介导的基因转染效率。肺癌细胞增殖特性的检测包括绘制细胞生长曲线、计算细胞群体倍增时间、流式细胞仪测定肺癌细胞的细胞周期分布。结果 :肺癌细胞的类型对脂质介导的基因转染效率具有显著影响。细胞增殖速度越快 ,处于分裂期 (G2 /M)的细胞越多 ,则脂质介导的基因转染效率越高。结论 :脂质介导的基因转染对细胞增殖的依赖 。Objective: To examine the relationship between the efficiency of cationic liposome-mediated transfection and the proliferation properties of lung cancer cells. Methods: Reporter gene expressing E.coli β-galactosidase and the eukaryotic expression vector,pCMV-cd, expressing E.coli cd,were used in order to evaluate transfection efficiency by histochemical staining and MTT assay respectively. The proliferation properties of cultured lung cancer cells were assessed by the cell growth curve of cultured cells by which the population doubling time was calculated and the cell cycle status was determined by flow cytometry. Results: The levels of efficiency of cationic liposome-mediated transfection were increased acomparided by the upregulation of proliferating rate of cultures and the number of cells in G 2/M phase. Conclusion: The dependence of liposome-mediated transfection on cell proliferation rate had an important implication that tissues consisting of rapidly proliferating cells would be more amenable to liposome-mediated gene therapy.

关 键 词：肺肿瘤 自杀基因 基因转移 阳离子脂质体 细胞增殖 基因治疗 

分 类 号：R734.2[医药卫生—肿瘤] R730.5[医药卫生—临床医学]