固体培养条件下氮源对Panus conchatus酶系及漆酶同工酶的影响  

Effects of Nitrogen Nutrient on Production of Lignocellulolytic Enzyme Profiles and Laccase Isoforms by Panus conchatus during the Solid State Fermentation

作　　者：胡平平[1] 付时雨[1] 余惠生[1] 

机构地区：[1]中国科学院广州化学研究所纤维素化学开放研究实验室,广州510650

出　　处：《纤维素科学与技术》2001年第1期1-7,共7页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金;广东省自然科学基金资助课题

摘　　要：在固体培养条件下 ,外加氮源对Panusconchatus产生木素降解酶有促进作用 ,其作用大小与含氮化合物的种类有关。研究发现在几种含氮化合物中 ,草酸胺对P .conchatus产木素降解酶的促进作用最显著 ,而对半纤维素酶 ,纤维素酶的产生促进作用不明显 ,在整个培养过程中 ,微晶纤维素酶活力都未检测到 ,CMC酶活力也较低。同时 ,还发现氮源对P .Nitrogen nutrient can stimulate the production of ligninolytic enzymes by Panus conchatus,the extent of stimulation was related to the sort of nitrogen-containing compounds.Ammonium oxalate among the used nitrogen-containing compounds had the most remarkable effect on the production of ligninolytic enzymes but no stimulating effect on the production of xylanase and cellulases by P.conchatus.During the whole culture,avicelase had not being detected,and CMCase had being kept very low.On the other hand,the effect of nitrogen nutrient on the production of laccase isoforms by P.conchatus has been studied,it showed nitrogen nutrient had a remarkable effect on the production of laccase isoforms by P.conchatus.

关 键 词：白腐菌 贝壳装革耳菌 木素降解酶 半纤维素酶 纤维素酶 同工酶 

分 类 号：Q55[生物学—生物化学]