烷化剂NTG诱变虾青素产生菌红法夫酵母的研究  

STUDY ON THE ASTAXAN TH IN-PRODUCING YEAST PHAFFIA RHODOZYMA MUTATED BY NTG

作　　者：吴江[1] 刘子贻[1] 朱寿民[1] 

机构地区：[1]浙江大学医学院生物化学教研室,杭州310031

出　　处：《微生物学通报》2001年第2期33-37,共5页Microbiology China

摘　　要：虾青素是一种很有效的生物抗氧化剂和某些生物的天然着色剂，应用前景广阔。红法夫酵母是 生产虾青素的一个来源，优点颇多。天然菌株虾青素产量较少，缺少实用价值。实验采用烷化剂ＮＴＧ 诱变红法夫酵母，筛选出类胡萝卜素产量高的诱变株。用薄层层析对红法夫酵母产生的色素及其皂 化产物进行分析，并对各个成分的扫描光谱进行了比较。认为红法夫酵母产生的类胡萝卜素成分主 要是虾青素及虾青素二酯，还有一部分β－胡萝卜素。同时，还对虾青素产生的时相和ＢＨＴ对虾青素 光分解的保护作用进行了初步研究。astaxanthin is an effective antioxidant and natural pigment which has wide application. Phaffia rhodozyma is a good source of astaxantin, but wild strain has limited use in industry because of low production level of astaxanthin. Several mutants of Phaffia rhodozyma were obtained by exerting mutagen N-methyl-N-nitro-N -nitrosoguanidine. The growth curve suggested that pigments were mainly produced in the middle and latter periods of log phase. The pigments were extracted from Phaffia rhodozyma and analysed by esterification, thin layer chromatography and absorption spectrometry. It was proved that astaxanthin, astaxanthin diester and β-carotene were the major components of the pigments produced by Phaffia rhodozyma. We also studied the pigments producing phase of Phaffia rhodozyma. and founded that astaxanthin was stable to light under butylated hydroxytoluene coexistance.

关 键 词：红法夫酵母 虾青素 N-甲基-N′-硝基-N-亚硝基胍 诱变 烷化剂 

分 类 号：Q939[生物学—微生物学]