P_(53)突变、表达及人乳头状瘤病毒感染与宫颈癌  

Study of HPV Infection, P_(53 ) Gene Mutation and Expression in Cervical Carcinoma

作　　者：李惠芳[1] 常艳丽[1] 李娜[1] 杨苏敏[2] 贾宗智[2] 

机构地区：[1]长治医学院中心实验室,长治046000 [2]长治医学院病理教研室,长治046000

出　　处：《生物化学与生物物理进展》2001年第2期214-217,共4页Progress In Biochemistry and Biophysics

基　　金：山西省自然科学基金!资助项目 (940 36 )&&

摘　　要：利用免疫组化、聚合酶链反应 (PCR)、单链构象多态性 (SSCP)分析等方法 ,对 49例同一标本宫颈癌组织中 p5 3蛋白、P53 外显子 7～ 8变异、HPV6、 11、 16、 18 DNA进行检测 ,以探讨它们在宫颈癌形成中的作用、相互关系和临床意义 .结果表明 :a P53 基因外显子 7～ 8突变率 14 2 9%、p5 3蛋白阳性率 48 98%、HPV DNA阳性率 87 76 % .b P53 基因突变不一定伴有 p5 3蛋白阳性 ,但P53 基因突变而 p5 3蛋白阴性的标本必是HPV DNA阳性 ;91 6 7%的 p5 3蛋白阳性标本具有HPV DNA阳性 .c HPV16 DNA阳性率显著高于HPV6、 11、 18 DNA阳性率 .证明 :宫颈癌的发生主要与HPV16感染有关 ,其次是P53 基因突变所致 ;p5 3蛋白阳性由HPV感染和 /或P53 基因突变所致 .P-53 gene (exon7 similar to8) mutatins and p53 proteins and HPV 6, 11, 16, 18-DNA were examined in 49 cervical carcinoma by immunohistochemistry, polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) in order to investigate their role and mutual relation and clinical significance in the oncogenesis of cervical carcinoma. The results showed that first, p53 proteins positive rate was 48.98%, and not outstandingly related to the differentiation and the invasive degree of cervical carcinoma (P>0.05); the defects of P-53 gene (exon7 similar to8) were not found but P-53 (exon7 similar to8) mutations were detected in 7 of 49 (14.29%) cervical carcinoma; then, HPV16-DNA positive rate was much higher than HPV6, 11, 18-DNA positive rate respectively (P<0.001), and the different HPV-DNA was simultaneously tested in one cervical carcinoma; last, not all cases of P53 mutations had p53 proteins positive, but the cases of P53 mutations and p53 proteins negative certainly had HPV infections, and HPV positive cases were much more than its negative one in the cases of p53 proteins positive (P<0.001). These results proved that the oncogenesis of cervical carcinoma is mainly associated with HPV16 infections, and second related to P-53 (exon7 similar to8) mutations. p53 proteins positive results from P-53 mutations or/and HPV infections in cervical carcinoma.

关 键 词：宫颈肿瘤 基因突变 P53 人乳头状瘤病毒感染 基因突变 

分 类 号：R737.330.2[医药卫生—肿瘤]