赤麂SRY基因的测序及其与部分偶蹄目动物SRY基因序列的比较  

SEQUENCING SRY GENE OF MUNTIACUS MUNTIAK VAGINALIS AND A PHYLOGENETIC COMPARISON WITH SOME OF ARTIODACTYLA

作　　者：郭金虎[1] 单祥年[1] 常青[1] 余多慰[1] 武景阳[2] 

机构地区：[1]南京师范大学生物学系,南京210097 [2]南京铁道医学院生物学教研室,南京210009

出　　处：《动物学报》2001年第2期145-149,共5页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目!(No . 3 9670 3 93 )&&

摘　　要：采用人SRY基因的一段保守序列的引物 ,通过PCR在雄性赤麂中扩增出了赤麂SRY基因的特异片段 ,通过DNA斑点杂交证实其扩增产物与人SRY基因有着较高的同源性。将PCR产物克隆到载体 pGEM TVec tor中 ,转化DH5α菌 ,经与人SRY基因探针进行菌落杂交筛选出赤麂SRY基因的阳性克隆 ,并对其进行了测序 .将其序列与基因库中录入的所有偶蹄目动物的SRY基因序列进行同源性比较 ,用UPGMA法构建了其系统进化树 ,从分类和进化上对赤麂SRY基因进行分析。With human SRY gene primers of the conserved region, the SRY gene fragment of M.m.vaginalis homologous to human SRY was amplified by PCR. In male M.m.vaginalis , a gene fragment of 221 bp homologous to human SRY was identified by DNA dot hybridization. By ligating pGEM T Vector with the PCR products of SRY gene of M.m.vaginalis and transforming the bacteria DH5α and clony hybridization with human SRY probe, the positive clony with M.m.vaginalis Sry gene fragment was identified gene, the SRY fragment of this clony was sequenced. The sequences identities of SRY gene fragments of all animals of Artiodactyla available from GenBank were compared with the M.m.vaginalis SRY gene fragment. The phylogenetic tree was contructed and analyzed by UPGMA method and it was studied at the level of taxonomics and evolution.

关 键 词：赤麂 SRY基因 性别决定 偶蹄目 DNA序列 系统进化 

分 类 号：Q343[生物学—遗传学]