番茄红素对人前列腺癌细胞(DU145)生长抑制的离体和整体水平研究  

Inhibition of lycopene on human prostatic cancer cells(Du145)in vitro and in vivo

作　　者：唐莉莉[1] 曾祥斌[1] 卢国栋[1] 金泰廙[1] 

机构地区：[1]复旦大学医学院毒理学研究室,上海200032

出　　处：《卫生毒理学杂志》2001年第1期1-4,共4页Journal of Health Toxicology

摘　　要：目的　研究番茄红素对人前列腺癌细胞DU145的影响。方法　体外培养的人前列腺癌细胞DU145经番茄红素作用后用苔盼蓝染色进行活细胞计数 ,绘制生长曲线并计算抑制率 ;应用流式细胞仪分析番茄红素对DU145细胞周期和凋亡的影响。制作裸鼠人前列腺癌模型 ,观察番茄红素在整体动物上的作用。结果　番茄红素可以明显抑制DU145细胞的生长 ,且呈剂量 效应和时间 效应关系。 10、2 0、40 μmol L处理细胞 7d后 ,细胞增长的抑制率分别为 10 76 %、92 90 %、99 11% (P <0 0 1)。裸鼠人前列腺癌模型上的研究显示 ,6 %番茄红素油树脂可显著抑制腺瘤的生长 ,30 0mg kg剂量组腺癌抑制率可达到 75 76 % (P <0 0 5 )。用经番茄红素处理过的细胞致瘤力消失。流式细胞仪分析显示番茄红素可影响该细胞周期并引起细胞凋亡 ,凋亡率高达 42 42 %。结论　番茄红素可以在离体细胞和整体动物水平明显抑制雄激素非依赖型人前列腺癌DU 145 ,其抑制机制可能是通过影响人前列腺癌细胞的生长周期和诱导其凋亡而实现。Objective The aim of this study was to investigate the influence of lycopene on human prostatic cancer cell line DU145.Methods Time and dose effects of lycopene were examined by using trypan blue exclusion staining;The influence on the cell cycle and the apoptosis were observed with flow cytometry.The effect of oral intake of lycopen on the growth of DU145 tumors in nude mice was observed.Results Lycopene could inhibit the growth of DU145 cell line with a dose\|effect and time\|effect relationship.The rate of cell growth inhibition was 10\^76%,92\^9% and 99\^11%( P <0\^01) in those cells treated with lycopene for 7 days.In vivo the significantly inhibitied growth( P <0\^05)was observed on the male BALB/C nude mice having DU145 tumors.The rate of inhibition was up to 75\^76% ( 300 mg/kg ).The lycopene\|treated cancer cells failed to form tumors in nude mice.Lycopene can influence the cell cycle of DU145 and induce apoptosis.Conclusion Lycopene inhibits DU145 cell line independent of androgen in \%vitro\% and in \%vivo\%,and the mechanisms may be related to its influence on the cell cycle and inducing of apoptosis.

关 键 词：番茄红素 细胞周期 凋亡 人前列腺癌细胞 裸鼠 

分 类 号：R737.250.2[医药卫生—肿瘤]