鱼肉中微囊藻毒素的高效液相色谱法分析  

Determination of Microcystins in Fish by High Performance Liquid Chromatography

作　　者：张维昊[1] 金丽娜[1] 徐小清[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《分析科学学报》2001年第2期117-119,共3页Journal of Analytical Science

基　　金：国家科技部和云南省政府招标课题<滇池蓝藻水华污染治理技术研究>(No.DCH-0 1 -0 0 1 )

摘　　要：应用反相高效液相色谱法分析了鱼肉中藻毒素的含量。用 BDSC1 8色谱柱 ,以水 (含 1 %三氟乙酸 )∶甲醇 =30∶ 70 ( V/V )溶液为流动相 ,2 38nm紫外检测。鱼肉样品用甲醇 -水和丙酮混合溶剂提取 ,经正己烷萃取后 ,将有机相弃去 ,水相用固相萃取柱净化后进行高效液相色谱分析。该法检测限为 1 0 ng/g,峰面积标准曲线在 5 0～ 2 5 0 ng范围内有良好线性关系 ,平均回收率为 85 .1 %～ 88.A reversed phased high performance liquid chromatographic method for the determination of microcystins in fish muscle is described. A BDS C18 column was used with a mobile phase of water (containing 1% trifluoroacetic acid)∶methanol =30∶70(V/V). The wavelength 238 nm was selected. The silver carp muscle sample was extracted with a mixture of methanol water and acetone. After extraction with hexane, the organic layer was discharged and the water phase was cleaned up using solid phased extraction columns before injection. The method is sensitive with detection limits of 10 ng/g, the linear range of the calibration curve of peak area was from 50 ng/g to 500 ng/g and the average recoveries of microcystins were 85.2%~88.1%.

关 键 词：反相高效液相色谱法 固相萃取 鱼肉 微囊藻毒素 污染物分析 水污染 湖泊 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] X524[轻工技术与工程—食品科学与工程]