用于分离EPA和DHA的新型HPLC固定相(Ⅰ)  

Novel HPLC stationary phase for separation of EPA and DHA (Ⅰ)

作　　者：李润[1] 张万宽[1] 陈德朴[1] 倪杭生[1] 王宏[1] 张秀荣[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《清华大学学报（自然科学版）》2001年第4期21-23,27,共4页Journal of Tsinghua University(Science and Technology)

摘　　要：为从鱼油中分离高纯度二十碳五烯酸 (EPA)和二十二碳六烯酸 (DHA) ,根据分子间的作用力与分子结构的关系 ,以及高效液相色谱 (HPL C)分离物质的原理 ,利用二乙烯苯交联的聚甲基丙烯酸甲酯 (PMDVB)树脂微球作为HPL C固定相 ,选择适当的流动相 ,从鱼油中分离 EPA,DHA,得到较好的分离效果。作者探索了微悬浮法合成PMDVB树脂的合成条件 ,并研究了交联度的大小和致孔剂组成对分离效果的影响。交联度为 35 % ,并具有特定孔结构的 PMDVB树脂微球作为 HPL C固定相 。Porous PMDVB resin modified with electron deficient 3, 5 dinitro benzoylchloride and 2 chloride ethanol was prepared and applied as high performance liquid chromatography (HPLC) stationary phases for the separation of EPA and DHA from fish oil. Satisfied separation results were obtained. Its column loading capacity, separation factor and column life were better than those of waters reverse phase column. When porous PMDVB resin modified with 3, 5 dinitro benzoylchloride was applied as a stationary phase, the most desirable separation results were obtained. Porous PMDVB resin modified with 2 chloride ethanol is cheap. So it is very possible for large scale application to separate EPA and DHA in industry. Discuss the separation mechanism, we found that as electron lacked group, 3,5 dinitro benzoylchloride combinated to EPA and DHA with tran electron complex action, which is the weak action among molecular.

关 键 词：EPA DHA HPL 分离 高效液相色谱固定相 二十碳五烯酸 二十二碳六烯酸 鱼油 聚甲基丙烯酸甲酯 

分 类 号：O657.7[理学—分析化学]