微卫星不稳定性-遗传性非息肉病性大肠癌诊断的分子标志:附4例报告  

MSI- a useful molecular indicator of Hereditary nonpolysis colorcctal cancer: a report of 4cases

作　　者：徐宁[1] 邱红明[1] 丁彦青[1] 徐莉[2] 

机构地区：[1]第一军医大学南方医院病理科,广州510515 [2]第一军医大学寄生虫学教研室,广州510515

出　　处：《第一军医大学学报》1999年第2期160-162,共3页Journal of First Military Medical University

基　　金：军队95攻关课题

摘　　要：目的探讨微卫星ＤＮＡ不稳定性（Ｍｉｃｒｏｓａｔｅｌｌｉｔｅ　ｉｎｓｔａｂｉｌｉｔｙ．ＭＳＩ）与遗传性非息肉病性大肠癌（Ｈｅｒｃｄｉｔａｒｙｎｏｎｐｏｌｙｓｉｓｃｏｌｏｒｅｃｔａｌｃａｎｃｅｒ，ＨＮＰＣＣ）的关系。方法采用银染ＰＣＲ－ＳＳＣＰ方法，检测４例遗传性非息肉病性大肠癌及其相应正常组织的第２、５、１７号染色体的４个位点的ＭＳＩ．结果４例ＦＩＮＰＣＣ均存在至少一个位点的ＭＳＩ。ＨＮＰＣＣ多位于右半结肠，低分化，粘液腺癌多见。结论ＭＳＩ是ＨＮＰＣＣ常见的分子学事件。ＨＮＰＣＣ有其特有的生物学特性。Objective To stlldy the relationship between microsatellite instability (MSI) and Hereditary nonpolysis colorectal cancer(HNPCC). Methods Silver staining PCR-SSCP method was used to detect MSI at 4 loci on chromosomes 2.5 and 17 in paraffinernbedded specimens of 4 HNPCC and their paired normal tissue. Results MSI was observed in all 4 HNPCC. It was predominantlya feature of right-sided tumor. of poorly differentiated mucous adenocarcinoma. Conclusion The results indicated that MSI is acommon molecular hereditary event in HNPCC. HNPCC has characteristic special biological feature.

关 键 词：MSI HNPCC 生物学特性 遗传性非息肉病性大肠癌 微卫星不稳定性 

分 类 号：R735.34[医药卫生—肿瘤]