马铃薯Y病毒HC-Pro中心区域在病毒协生作用中的主导地位  

The Primary Role of Central Region of HC-Pro of Potato Y Potyvirus in Synergism of Plant Viruses

作　　者：鲁瑞芳[1] 李为民[1] 王海云[1] 郭明[1] 彭学贤[1] 

机构地区：[1]中国科学院微生物研究所植物生物技术开放实验室,北京100080

出　　处：《生物工程学报》2001年第3期264-268,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金! ( 3980 0 0 0 1)&&

摘　　要：利用PCR方法获得了马铃薯病毒中国株系 (PVY C)HC Pro基因的 5个缺失突变体 ,构建了相应的植物表达载体。通过土壤农杆菌 (Agrobacteriumtumefaciens)介导法转化了烟草品种K32 6 (Nicotinatabacumcv.K32 6 )。PCR和Southernblot分析证明了HC Pro基因及其缺失突变体已整合到烟草基因组中 ,Westernblot表明它们在转基因烟草中得到了表达。侵染性试验发现HC Pro中心区域介导转基因烟草中PVY C和黄瓜花叶病毒 (CMV)、PVY C和马铃薯X病毒 (PVX)之间的协生作用 ,从而明确了PVY CHCFive deleted mutants of HC\|Pro gene of Chinese isolate of potato Y potyvirus (PVY\|C) were obtained by PCR mutation,and their plant expression vectors were constructed.They were transformed into tobacco K326 ( Nicotina tabacum cv.K326) mediated by Agrobacterium. PCR and Southern blot analysis revealed that PVY\|C HC\|Pro gene and its deleted mutants were integrated into tobacco genome,and Western blot analysis showed that they were all expressed in transgenic tobacco plants.Furthermore,infection test demonstrated that the central region of PVY\|C HC\|Pro can mediate synergism of PVY\|C/cucumber mosaic cucumovirus (CMV) and PVY\|C/potato X potexvirus (PVX),identifying that it is functional domain in synergism.

关 键 词：马铃薯Y病毒 HC-PRO基因 突变 中心区域 协生作用 

分 类 号：S432.41[农业科学—植物病理学] S435.32[农业科学—农业昆虫与害虫防治]