IL-1β对大鼠胆碱能神经元的影响  

Effect of IL-1β on cholinergic neurons in the rat

作　　者：刘恒颖[1] 康劲松[1] 徐琪[1] 郭中钰[1] 祝世功[1] 

机构地区：[1]吉林大学基础医学院病理生理教研室,吉林长春130021

出　　处：《中风与神经疾病杂志》2001年第2期87-88,共2页Journal of Apoplexy and Nervous Diseases

摘　　要：目的　探讨 IL- 1β对大鼠胆碱能神经元的损伤作用及机制。方法　将 IL- 1β缓慢释放颗粒 (IL- 1βpellet)植入大鼠右侧 Meynert核 (nb M,基底核 ) ,复制胆碱能系统受损的动物模型 ;将不同剂量的人重组白细胞介素 - 1β(rh IL- 1β)加入培养大鼠前脑基底神经元中 ,复制 rh IL- 1β诱导神经元损伤模型 ,检测 (乙酰胆碱酯酶 ) Ach E活性和脂质过氧化物 (L PO)含量的变化。结果　 IL- 1β损伤侧皮质 Ach E活性较未损伤侧、正常组及假手术组均显著降低 (P <0 .0 1)。 5 0 - 5 0 0 U/ ml rh IL- 1β可引起培养的神经元 Ach E活性明显下降 (P <0 .0 5 ) ;10 0 - 5 0 0 U/ ml rh IL-1β可引起神经元 L PO含量明显升高 (P <0 .0 1)。结论　 IL- 1β慢性作用可以造成大鼠中枢胆碱能系统的损害 。Objective To observe the effect and mechanism of IL 1β on cholinergic neurons in the rat. Methods IL 1β pellet was put into right side of nucleus basalis of Meynert to make cholinergic system damaged. Different doses of rhIL 1β were added into cultured forebrain neurons to make cultured neuron damaged. AchE activity and lipid peroxide(LPO) content were measured respectively. Results In the rat model AchE acitivity in cerebral cortex was lowered significantly. In cultured neuron 50～500 U/ml doses of rhIL 1β reduced AchE acitivity and 100～500U/ml increased LPO content. Conclusion Chronic stimulation of interleukin 1 can damage central cholinergic neurons and its projectile system by one way of neuronal peroxidization.

关 键 词：IL-1Β 缓慢释放颗粒 人重组白细胞介素-1β 乙酰碱酯酶 脂质过氧化物 MEYNERT基底核 AD 阿尔茨海默病 

分 类 号：R749.16[医药卫生—神经病学与精神病学] R338[医药卫生—临床医学]