牛磺酸减少大鼠肠套叠的发生  

Taurine decreases the incidence of intussusception in the rat

作　　者：王平[1] 雷宇[1] 佟利家[2] 王晓红[2] 齐永芬[2] 唐朝枢[2] 

机构地区：[1]北京大学第一医院小儿外科,北京100034 [2]北京大学第一医院心血管病研究所,北京100034

出　　处：《北京大学学报（医学版）》2001年第1期35-37,共3页Journal of Peking University:Health Sciences

摘　　要：目的 :在细菌脂多糖 (LPS)诱导的大鼠肠套叠模型上 ,观察牛磺酸在肠套叠发生中的作用。方法 :高脂高糖喂养大鼠后 ,腹腔注射LPS制作肠套叠模型 ;Greiss法测定血浆和结肠平滑肌组织硝酸盐和亚硝酸盐 (NOx)的含量 ,放射免疫法测定血浆和组织环磷酸腺苷 (cGMP)含量。结果 :LPS( 10mg/kg .ip)处理后 6h诱发大鼠肠套叠发生率达 40 %。应用LPS的同时给予牛磺酸 ,肠套叠的发生率为 16 .7% ,与单纯脂多糖组相比下降 5 8.2 5 % (P <0 .0 1)。大鼠给予LPS ,无论是否发生肠套叠 ,血浆和结肠平滑肌组织NOx、cGMP含量均升高。结肠平滑肌组织总一氧化氮合酶 (tNOS)、诱导型一氧化氮合酶 (iNOS)增高 ,而内皮型一氧化氮合酶 (eNOS)下降。大鼠给予牛磺酸后 ,血浆和结肠组织NOx、cGMP的含量分别降低 2 3 .7% (P <0 .0 1) ,2 0 .6 % (P <0 .0 1)和 37.2 % ,2 2 .9% (P <0 .0 1)。结肠组织的tNOS、iNOS的活性分别降低 2 3 .2 %和 2 4.2 % (P <0 .0 1) ,而cNOS的活性增高 5 7.1% (P <0 .0 1)。结论 :牛磺酸可减少肠套叠的发生 。Objective: In this experiment, a rat intussusception(IN) model induced by lipopolysaccharide(LPS) was used to study the effect of taurine on the occurrence of IN. Methods: Preparation of IN model; NO x in plasma and colic smooth muscle was measured by Griess method; cGMP content and NO synthase(NOS) activity were measured by radioimmunoassay. Results:LPS(10 mg/kg.ip)caused IN in up to 40% of the rats 6 h after treatment of LPS, but administration of taurine concurrently with LPS reduced the IN incidence by 16.7%. NO x and cGMP contents in plasma and colic smooth muscle were significantly increased in both IN and non IN rats 6 h after administration of LPS, in addition,total NOS(tNOS) and inducible NOS (iNOS) activities in colic smooth muscle were also increased, but constitutive NOS(cNOS)activity was decreased. Treatment of taurine at the time of LPS, the contents of NO x and cGMP in plasma and colic smooth muscle were decreased by 23.7%( P <0.01), 20.6%( P <0.01) and 37.2%( P <0.01), 22.9%( P <0.01),respectively, and the tNOS and iNOS activities were reduced by 23.2% and 24.2 % ( P <0.01),respectively, but cNOS activity was increased by 57.1%( P <0.01). Conclusion:Taurine could lower the incidence of IN of rats, which might be related to its inhibition of NO production through different pathway.

关 键 词：肠套叠 牛磺酸 一氧化氮合酶 

分 类 号：R574.3[医药卫生—消化系统]