番鸭细小病毒弱毒株VP_2基因的分子克隆与序列测定  

Molecular Cloning and Sequencing of DNA Encoding VP_2 structural Protem on Attenuated strain of Muscovy Duck Parvovirus

作　　者：娄华[1] 白挨泉[1] 陆英杰[1] 顾万军[1] 贺东升[2] 刘福安[2] 

机构地区：[1]佛山科技学院动物医学系,广东南海528231 [2]华南农业大学动物医学系,广东广州510642

出　　处：《中国兽医科技》2001年第6期3-5,共3页Chinese Journal of Veterinary Science and Technology

摘　　要：将番鸭细小病毒 (MDPV)强毒Q株经番鸭胚连续传代 ,获得致弱株MDPV 2 6。根据已发表的MDPV的全基因序列 ,设计了 1对引物LHMP7/LHMP8,同时在这 2条引物中分别加入 2种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点。应用PCR技术扩增了MDPV 2 6株的VP2 基因片段。将扩增后的基因片段重组到 pMD18 T质粒载体上 ,并对插入片段进行序列测定。测序结果表明 ,弱毒株MDPV 2 6与强毒株MDPV Q的VP2 基因序列相同率达 99.7% ,与国外分离株的同源性为 98.3 % ,说明强、弱毒株的VP2The attenuated strain MDPV 26 had been derived by continued passage of virulent wild type (MDPV Q) in muscovy duck embryo. According to reported complete nucleotide sequences of Muscovy duck parvovirus, two modified primers (LHMP7/LHMP8) were designed and for each of them, a restriction endonuclease recognition site, SacⅡ or KpnⅠ, was included respectively. The DNA encoding VP 2 structural protein of MDPV 26 was amplified by PCR. The amplified DNA of MDPV 26 was cloned into pMD18 T vector and sequenced. Sequence indicated that both DNAs encoding VP 2 structural protein of MDPV Q and MDPV 26 displayed 99.7% identity; and share 98.3% homology with that of reported MDPV.

关 键 词：番鸭细小病毒 弱毒株 分子克隆 序列测定 VP2基因 免疫原性 

分 类 号：S852.659.2[农业科学—基础兽医学] Q78[农业科学—兽医学]