8种人恶性淋巴瘤细胞系P16基因突变的研究  

Mutations of P16 Gene in 8 Human Malignant Lymphoma Cell Lines

作　　者：杨年兰[1] 陈燕[2] 李崇渔[2] 

机构地区：[1]武汉大学人民医院血液科,武汉430060 [2]华中科技大学同济医学院附属协和医院血液科,武汉430030

出　　处：《同济医科大学学报》2001年第3期229-231,共3页Acta Universitatis Medicinae Tongji

基　　金：国家教委留学回国人员启动基金(No. 1995 - 135 )

摘　　要：为确定 P16基因与恶性淋巴瘤发生发展的关系 ,对 8种人恶性淋巴瘤细胞系中 P16基因突变情况进行研究。用银染 PCR- SSCP方法对 P16基因第二外显子突变情况进行研究 ,并对检出突变者用自动测序仪进行测序分析。检出1例纯合缺失 ,2例 P16基因第二外显子突变。测序分析发现 SU- DHL- 1P16基因第二外显子有 3处点突变 ,即密码子72的 GAC突变为 AAC,密码子 77的 GCT突变为 ACT,密码子 82与 83之间插入了一个 C。认为 P16基因在 B细胞恶性淋巴瘤细胞系中的突变率较高 ,提示其改变可能参与恶性淋巴瘤的发生发展。In order to determine the relationship between P16 and the tumorigenesis of malignant lymphoma, silver stained PCR SSCP analysis and direct sequencing were used to evaluate the alterations of P16 gene in 8 human malignant lymphoma cell lines. A homolygous loss was found in SU DHL 9 while mutations of P16 exon 2 in SU DHL 1 and Daudi. Sequencing of the PCR product showed three point mutations in SU DHL 1, which were GAC to AAC in codon 72, GOT to ACT in codon 77, and a insertion of C between codon 82 and 83, respectively. This study suggested the mutations of P16 gene are highly frequent in B cell lymphoma cell lines and may play an important role in its tumorigenesis.

关 键 词：P16基因 恶生淋巴瘤 基因突变 

分 类 号：R733.4[医药卫生—肿瘤] R730.231[医药卫生—临床医学]