幽门螺杆菌热休克蛋白A亚单位基因工程菌的发酵  

Fermentation of Gene Engineering E . coil of Helicobacter pylori Heat Shock Protein A

作　　者：王缚鲲[1] 张卫军[1] 邹全明[1] 郭学青[1] 于长青[1] 曾浩[1] 

机构地区：[1]第三军医大学临床微生物教研室,重庆400038

出　　处：《中国生物制品学杂志》2001年第2期84-86,共3页Chinese Journal of Biologicals

基　　金：国家"九五"重点科技攻关项目(96-901-01-54)。

摘　　要：目的 研究幽门螺杆菌热休克蛋白Ａ亚单位（ＨｓｐＡ）基因工程菌的发酵工艺。方法 通过不同培 养基、不同葡萄糖浓度、不同溶氧条件、补料与非补料对幽门螺杆菌热休克蛋白Ａ亚单位基因工程菌的菌体生长与 外源蛋白表达量的影响的比较，确定其较为合适的培养条件。结果 多批实验证明，菌体单产可达４２ｇ／Ｌ，目的蛋 白的表达率为３８．５％。结论 此发酵工艺可以提高ＨｓｐＡ基因工程菌菌体的得率和目的蛋白的表达。Objective To investigate fermentation conditions of gene engineering E. coli strain for expression of Helicobacter pylori heat-shock protein A( HspA). Methods The influences of media, glucose concentration, dissolved oxygen and fed-batch on the growth of gene engineering E. coli strain and expression of Hsp A were compared, and, on the basis of this, the optimal fermentation conditions were studied. Results Under the optimal fermentation condition, the cell output and expression rate of Hsp A reached 42g/L and 38. 5% respectively. Conclusion Both the recovery rate of gene engineering E. coli strain and expression level of Hsp A were improved under the fermentation condition.

关 键 词：幽门螺杆菌 热休克蛋白A亚单位 大肠杆菌 发酵工艺 

分 类 号：R378.2[医药卫生—病原生物学]