蜜环菌胞外漆酶酶活力的分光光度法测定  

Spectrophotometric Determination of Extracellular Laccase Activity of Armillaria Mellea

作　　者：吴涓[1] 肖亚中[1] 王怡平[1] 李清彪[2] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230039 [2]厦门大学化学工程系,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2001年第4期893-898,共6页Journal of Xiamen University：Natural Science

基　　金：安徽省科委自然科学基金资助项目 (982 12 4 11)

摘　　要：采用分光光度法测定蜜环菌 (Armillaria mellea)胞外漆酶 (L accase)的活力 ,分别以 2 ,2’-连氮 -二 (3-乙基苯并噻唑 - 6 -磺酸 ) (简称 ABTS)、丁香醛连氮、愈创木酚和邻联甲苯胺为底物 ,比较了这 4种底物对漆酸的敏感性 ,探讨了酶反应时间、温度、缓冲液及其 p H值和底物与酶的用量等条件对漆酶催化反应的影响 .实验结果表明 。Spectrophotometric determination was taken to determine extracellular laccase activity from Armiillaria mellea . ABTS, syringaldazine, guaiacol and tolidine was used as the substrate for laccase respectively. The effects of time, temperature, buffer and its pH, and the amount of substrate and enzyme on laccase catalytic reaction were studied. The experimental results showed that suitable determination could ensure rapid and accurate determination of laccase activity.

关 键 词：漆酶 底物 酶活力 蜜环菌 分光光度法 测定 

分 类 号：Q554[生物学—生物化学]