不同剂量^(60)Co辐照血红细胞CR_1分子定量与基因表达  

The ECR1genomic density polymorphism and quantitative expression after various doses of ^(60)Co Γ radiation blood

作　　者：李志强[1] 徐文皓[1] 乐嘉宜[2] 徐钧[1] 陈卫宾[1] 罗丽芳[1] 

机构地区：[1]上海市第六人民医院,200233 [2]上海市黄浦区中西医结合医院

出　　处：《上海医学》2001年第5期278-280,共3页Shanghai Medical Journal

基　　金：上海市卫生局科技发展基金项目资助 (编号 9943 2 )

摘　　要：目的　研究不同剂量60 Co射线辐照离体全血对红细胞CR1分子基因表达与分子数目的影响。方法　应用PCR和HindIII酶切技术测定红细胞CR1分子基因表达以及应用酶联法定量测定红细胞CR1分子数目。结果　经 15～ 35GY60 不同剂量辐照离体全血红细胞CR1分子基因表达与未经照射组比较无明显差异 (P>0 .0 5 )。经 15～ 2 5GY辐照后红细胞CR1分子数目与未经照射组比较无明显差异 (P >0 .0 5 ) ;然而 ,经 30、35GY辐照后红细胞CR1分子数目分别与其他剂量组和未经照射组比较有明显差异 (P <0 .0 5～ 0 .0 1)。另外 ,各剂量组、未经照射组高表达离体全血红细胞CR1分子数目相互比较无明显差异 (P >0 .0 5 ) ;且中低表达离体全血相互比较也无明显差异 (P >0 .0 5 )。各剂量组、未经照射组高表达与中低表达离体全血红细胞CR1分子数目比较则有明显差异 (P <0 .0 5～ 0 .0 1)。结论　在一定照射剂量范围内 ,不影响红细胞CR1分子基因表达 ;随着照射剂量逐渐增大 ,离体全血红细胞CR1分子数目逐渐升高且呈正相关。Objective To study the effect of complement receptor type 1 (CR1) on erythrocytes genomic density polymorghism and quantitative expression after various doses of 60 Co г radiation. Methods Methods Polymerase chain reaction (PCR), Hind III restricton enzyme digestion and the quantitaive assay of ECR1 were used. Results From 15 Gy to 35 Gy 60 Co г radiation,the ECR1 genomic density polymorphism had no obvious differences between radiation groups and unradiation group. ( P >0.05); From 15Gy to 25Gy, the quantitative expression in RBC was very similar among radiation groups and unradiation group ( P >0.05); But after 30Gy and 35Gy 60 Co radiation, the quantitative expression had obvious difference ( P <0.05～0.01).On the other hand, the high and middle low quantitative expression in RBC had no obvious difference from 15Gy to 35Gy ( P >0.05), and it also had no obvious difference among the radiation group and unradiation group ( P >0.05). But in every doses group and unradiation group, there were obvious difference among high expression and middle low expression( P <0.05-0.01). Conclusion Within limited doses of radiation, it the erythrocyte (CR1)gene expression is not influenced; with the increasing with the of radiation dose,the quantitative expression in RBC is also increased.

关 键 词：辐照剂量 红细胞 受体 补体 基因表达 CR1 钴60 血液制品 

分 类 号：R817[医药卫生—影像医学与核医学]