先天性巨结肠病肠壁NOS阳性神经元光镜和电镜观察  

OBSERVATIONS ON THE NOS POSITIVE NEURONS IN HIRSCHSPRUNG DISEASE BY LIGHT AND ELECTRON MICROSCOPY

作　　者：章明[1] 潘剑威[1] 任天荣[1] 韩永坚[1] 章锁江[2] 钟海玲[3] 熊启明[4] 

机构地区：[1]浙江大学医学院解剖学教研室,杭州310031 [2]浙江大学医学院病理学教研室,杭州310031 [3]浙江大学医学院中心实验室,杭州310031 [4]浙江大学医学院附属儿童医院,杭州310031

出　　处：《解剖学报》2001年第3期216-219,共4页Acta Anatomica Sinica

基　　金：浙江省测试基金资助项目 (960 5 2 )

摘　　要：目的　探讨NOS阳性神经元与先天性巨结肠病病因及病理机制的关系。　方法　对扩张段和移行段肠壁分别作全层铺片 ,NADPH d酶组织化学染色 ,光镜和扫描电镜下观察NOS阳性神经结构。　结果　扩张肠段光镜下肠肌丛神经节和神经元均较大 ,节内神经元染色深数量多 ,沿神经节周边及神经纤维发出处排列。扫描电镜下神经元胞体较大 ,排列较密 ,发出的神经纤维较多 ,在各个方向上相互连接。沿肌纤维排列的神经元之间有较多的横向连接纤维 ,肠肌丛神经元还通过穿行于环行肌层的神经纤维和粘膜下层神经元相连接。移行段光镜下节内神经元胞浆染色较淡 ,深浅不一 ,神经节和神经元均较小 ,发出的纤维较细且染色较淡。扫描电镜下神经元胞形较小 ,且大小不等 ,密度较小 ,神经元间的纤维联系及神经纤维攀附于肌纤维表面的现象均较少。神经元和神经纤维呈沿纵行肌长轴线性分布。Objective To explore the etiology and pathogenesis of Hirschsprung disease(HD) and the role of nitric oxide in pathophysiology of HD. Methods The whole amount preparations of dilative and transitional segments were stained by NADPH\|diaphorase histochemistry mothod,and colonic walls were digested by dispase. Then the NOS positive nervous structure were observed by light and scanning electron microscope. Results In dilative segment, under light microscope, ganglion and neuron were bigger and more darkly stained. Intraganglionic neurons distributed mostly in peripheral area of ganglion and basal part of ganglionic fiber bundles. Under scanning electron microscope, the neurons were denser, more nerve fibers from them and connected with each other in all dimensions. And more transverse connected fibers between neurons which were arranged along muscle fibers. Myenteric plexus even connected with the submucosal plexus by nerve fibers which passed through circular muscle layer. In transitional segment, under light microscope, intraganglionic neurons cytoplasm were lightly stained and also variedly. The ganglion and neurons of the segment were smaller, and the fibers from them were thinner and paler than those of the dilative segment. Under scanning electron microscope, neurons' density was lower, the fiber connection between neurons and muscle fibers/neurons was lesser. In the transitional segment, neurons and nerve fibers were distributed linearly along the longitudinal muscle fibers. Conclusion Our results indicated that an intimate relationship existed between the development of Hirschsprung disease and abnormal distribution and metabolism of NOS positive neuron in colonic wall. [

关 键 词：先天性巨结肠病 NOS阳性神经元 光镜 扫描电镜 

分 类 号：R574.62[医药卫生—消化系统] R656.9[医药卫生—内科学]