β-胡萝卜素抑制人肝癌细胞株SMMC-7721增殖的研究  

Inhibitory effects of β-carotene on hepatic cancer cell line SMMC-7721

作　　者：骆文静[1] 王文亮[2] 李泊[1] 陈耀明[1] 缪珊[1] 

机构地区：[1]第四军医大学营养与食品卫生学教研室,西安710033 [2]第四军医大学基础部病理学教研室

出　　处：《卫生研究》2001年第4期213-214,共2页Journal of Hygiene Research

摘　　要：为了探讨 β 胡萝卜素抑制肝癌细胞增殖的机制 ,在体外培养肝癌细胞株SMMC 772 1,培养基添加2 0、40及 80 μmol Lβ 胡萝卜素作用 12、2 4和 48h后 ,用四唑盐比色试验 (MTT)检测细胞的存活和生长 ;台盼蓝拒染法绘制生长曲线 ;DNA凝胶电泳检测肝癌细胞凋亡。MTT检测结果显示 2 0～ 80 μmol L的 β 胡萝卜素均对SMMC 772 1细胞株有显著的抑制作用 ,且呈剂量 效应关系。DNA凝胶电泳结果显示 β 胡萝卜素能够诱导SMMC 772 1细胞凋亡。结论认为 β 胡萝卜素对人肝癌细胞株SMMC 772 1的增殖具有显著的抑制作用 。The inhibitory effect of β carotene on the proliferation of hepatic cancer was studied. Cells from a hepatic cancer cell line SMMC 7721 were incubated in culture media with 20, 40 and 80μmol/L β carotene for 12, 24 and 48h respectively. MTT test, Trypan blue exclusion test and DNA gel electrophoresis were used. The results of MTT test revealed that β carotene (20—80μmol/L) could inhibit the proliferation of SMMC 7721 cells in a dose dependent manner. DNA gel electrophoresis showed that the apoptosis of hepatic cancer cells could be induced by β carotene (40μmol/L). It is concluded that the proliferation of hepatic cancer cells inhibited by β carotene, probably through interfering DNA metabolism and inducing cell apoptosis.

关 键 词：Β-胡萝卜素 肝癌 细胞凋亡 细胞周期 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤] R977.21[医药卫生—临床医学]