原发性膀胱移行细胞癌中p16基因状态的研究  

The status of p16 gene in primary transitional cell carcinoma of bladder

作　　者：胥飙[1] 邓远忠[2] 

机构地区：[1]重庆医科大学临床学院检验科,重庆400016 [2]重庆医科大学临床学院泌尿科,重庆400016

出　　处：《重庆医科大学学报》2001年第3期250-252,共3页Journal of Chongqing Medical University

摘　　要：目的：研究原发性膀胱移行细胞癌中ｐ１６基因的状态。方法：采用多重ＰＣＲ分析法、ＳＳＣＰ分析法及以ＰＣＲ为基础的甲基化分析法，分别对４０例原发性膀胱移行细胞癌及其相应的癌旁组织中ｐ１６基因的缺失、突变及甲基化情况进行了检测。结果：４０例肿瘤标本中，发现７例（１７．５％）ｐ１６基因缺失，３例（７．５％）发生了突变，９例（２２．５％）有甲基化存在。全部相应的癌旁组织中均未发现有ｐ１６基因缺失、突变或甲基化存在。结论：ｐ１６基因的异常改变在原发性膀胱移行细胞癌的发生与发展中可能起重要作用。Objective： To investigate the status of p16 gene in primary transitional cell carcinoma of bladder(TCCB).Methods： A total of 40 TCCB samples with correspondent tumor-adjacent specimens were screened for p16 gene deletions,mutations and hypermethylations by using multiplex polymerase chain reaction(PCR), single strand conformational polymorphism (SSCP) and PCR-based methylation analysis,respectively. Results： Among 40 TCCB samples,7 deletions ， 3 mutations and 9 hypermethylations were detected,and their rates were 17.5%,7.5% and 22.5% ,respectively. However,no deletions,mutations or hypermethylations were found in all the tumor-adjacent tissues.Conclusion： The abnormal alterations of p16 gene may play an important role in the carcin ogenesis of TCCB.

关 键 词：膀胱癌 P16基因 缺失基因 突变基因 甲基化 

分 类 号：R737.14[医药卫生—肿瘤]