钩藤对致痫大鼠海马脑片诱发场电位的影响  

EFFECT OF UNCARIA ON EVOKED FIELD POTENTIAL OF HIPPOCAMPAL SLICE IN EPILEPTOGENIC RAT

作　　者：徐淑梅[1] 何津岩[1] 林来祥[1] 郑开俊[1] 仇晓菁[1] 

机构地区：[1]天津医科大学生理教研室,天津300070

出　　处：《中国应用生理学杂志》2001年第3期259-261,共3页Chinese Journal of Applied Physiology

基　　金：天津市 2 1世纪 -青年科学基金资助项目(96 3 70 8411)

摘　　要：目的 :研究钩藤对癫痫模型海马脑片诱发场电位的影响。方法 :以毛果芸香碱致痫大鼠为实验对象 ,采用脑片旁滴注给药 ,用细胞外玻璃微电极记录方法 ,观察钩藤对癫痫模型离体海马脑片CA1区锥体细胞诱发群锋电位(populationspike,PS)的影响。结果 :给予钩藤后使致痫大鼠海马脑片PS幅度平均降低 2 7.64% ,平均 8.71min恢复(n =14,P <0 .0 1)。结论 :钩藤能降低致痫大鼠海马脑片CA1区顺向诱发PS幅度 ,提示钩藤对中枢神经系统的突触传递过程有明显的抑制效应 。Aim: To investigate the effect of uncaria on evoked field potential of CA1 hippocampal slice in epileptogenic model. Methods: We made rats which were injected by pilocarpine as epileptic models. The pathway of drug administration is titration adjacent to hippocampa1 slices. We recorded the evoked field potential of hippocampal slice by glass microelectrode extracellularly, and observed the effect of uncaria on the amplitude of population spike (PS) in CA1 pyramidal cells in hippocampal slices.Results: The uncaria with concentration of 1 g/ml could reduce the amplitude of PS in CA1 of hippocampal slices. The average of fall was 27.64%,and restored in 8.71 minutes on average ( n =14, P < 0.01 ).Conclusion: Uncaria could reduce the amplitude of PS in CA1 of epileptic hippocampal slices remarkably.These results show that uncaria can inhibite the synaptic transfer activity of CNS remarkably, suggest that it has the distinct effect of anti epilepsy

关 键 词：钩藤 癫痫 海马脑片 群锋电位 药理 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R971.6[医药卫生—临床医学]