实验大鼠反复脑缺血再灌流海马活体透析氨基酸、单胺递质及其代谢产物的研究  

Effect of Repeated Global Ischemia by in vivo Dialysis on Extracellular Concentrations of Neurotransmitter Amino Acids, Dopamine, Serotonin and Their Metabolities in Rat Hippocampus

作　　者：贾健民[1] 申玉[1] 贾琳[1] 贾健平 M Pollock 

机构地区：[1]邯郸市第一医院神经内科,056002 [2]Otago大学医学院神经内科

出　　处：《中国临床神经科学》2001年第2期139-141,145,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的 :研究脑反复缺血后海马细胞外液氨基酸和单胺递质及其代谢产物的变化规律。方法 :采用Pulsinelli和Brierley四血管关闭的方法 ,使大鼠脑反复缺血。用海马微管透析及高效液相电化学检测细胞外谷氨酸 (Glu) ,天冬氨酸 (Asp) ,牛磺酸 ,丙氨酸 ,丝氨酸 ,多巴胺 (DA) ,5 羟色胺 (5 HT)及其代谢产物浓度的变化。结果 :缺血期 ,Glu和Asp骤然增高 5 0倍和 3 0倍。缺血期间DA和 5 HT含量分别增加 3 0倍和 5 0倍 ,随后逐渐下降 ,再灌流 10 0min恢复到基础值水平。与此同时 ,它们的酸性代谢产物 3 ,4二羟苯乙酸 (DOPAC) ,高香草酸 (HVA) ,5 羟吲哚乙酸 (5 HIAA)在缺血期明显下降。结论 :缺血期间海马细胞外液兴奋性氨基酸和单胺递质急剧大量释放并触发膜离子通道改变 ,Ca2 + 超载 ,自由基反应 。Aim:To study the cause of the vulnerability of hippocampus to ischemia, it is important to explore the effects of monoamine and excitatory amino acids on hippocampus. Methods:Following repeated global ischemia induced by Pulsinelli and Brierley's 4 VO (4 vessel occlusion) animal model in the rat, hippocampal extracellular fluid levels of monoamine and excitatory amino acids were determined at different time by using intracerebral microdialysis, high performance liquid chromatography and electrochemical detection (HPLC ECD). Results:Compared with pre ischemic level, there was a large increase of extracellular DA (dopamine) (30 fold) and 5 HT (50 fold) and excitatory amino acids during period of ischemia (50 fold for Glutamate, 30 fold for Aspartate, and 1 8 fold for Serine) and reperfusion (2 3 fold for Taurine, 1 5 2 0 fold for Alanine, and 2 fold for Serine) with a accompaniment of a decrease in levels of DOPAC, HVA, and 5 HIAA, and a returned normal level of DA and 5 HT as those of DOPAC, HVA, and 5 HIAA after 100 min reperfusion. Conclusion:The neurotoxin, overloaded calcium, free radical reaction, as a sequence of large increase in the contents of extracellular monoamines and excitatory amino acids in the hippocampus induced by ischemia, are all contributory to the vulnerability of hippocampus to ischemia.

关 键 词：微透析 海马 多巴胺 5-羟色胺 氨基酸 脑缺血 再灌注损伤 大鼠 单胺递质 

分 类 号：R743.31[医药卫生—神经病学与精神病学]