凝固酶及核酸酶试验综合评价金黄色葡萄球菌致病力的实验研究  

Coagulase and Nucleinase tests general evalulation trial study of stophylococcus aureus Pathogenicity Health Prevention Station of MaAnShan AnHui Chen DaoLi Gao Zheng Huo KaiLan Liu Yan

作　　者：陈道利[1] 高峥[1] 霍开兰[1] 刘燕[1] 

机构地区：[1]安徽省马鞍山市疾病预防控制中心,安微马鞍山242700

出　　处：《中国卫生检验杂志》2001年第3期257-258,共2页Chinese Journal of Health Laboratory Technology

摘　　要：〔目的〕本文通过简便、快速、成本低廉的耐热核酸酶测定 ,反映金黄色葡萄球菌产生肠毒素情况 ,并结合血浆凝固酶综合评价其致病力。〔方法〕耐热DAN酶试验 ,从不同来源样品中分离出的 36株试验菌与标准株 (6 5 38- 95 )在甲苯胺蓝核酸琼脂培养基 (Tris.DAN)上打两排孔 ,一组经加热处理的培养物 ;另一组加未经热处理培养物 ,37℃ 3h观察结果。血浆凝固酶试验 :用 1:4新鲜兔血浆按食品卫生检验标准 (GB4789.10— 94.6 .1.5 )进行操作。〔结果〕136株菌血浆凝固酶与耐热核酸酶阳性符合率为 90 .4%,其中 85 %菌株可引起食物中毒。〔结论〕这次实验研究说明了耐热核酸酶与血浆凝固酶二者结果反映出该菌引起食物中毒的可能程度 ,适合于目前我国多数基层实验室开展 ,为卫生监督执法提供准确可靠的依据。Object to examine thermonuclease of convenient, fasten, cost inonpensive, refiect out stophylococcus aueus turn out enterotoxin, and connect with coagulase general evaluation pathogenincity. 〔Methods〕 thermonuclease trial:In differently source specinen division 136 case trial strains and standard strains( 6538-95) culture on Tolnidine Blue DAN agar culture media. To take two group hole in Toluidine Blue DAN Agra cluture media, a group take heating culture liquid, the other take unherrting culture liquid. Taking in 37 centigrade warmtank culture period three hour. Coagulase trial: Useful 1:4 fresh rabbit serum take on operation in line with Foods Health Examine Standard (GB4789.10—94.6.1.5).Results: 136 Strains coagulase and thermonuclease positive ration ninety point foure precent, eighty and five precent strains can take on foods toxication. 〔Conclusion〕 The two methods of thermonuclease and coagulase accout for arising foods toxication probability degrec.To adpt tomyprovince in allbase lab work in present. For Health legalist and monitor based on precisely andfaithfu.

关 键 词：肠毒素 食物中毒 耐热核酸酶 基层实验室 

分 类 号：R155.3[医药卫生—营养与食品卫生学] R117[医药卫生—公共卫生与预防医学]