结肠直肠癌患者PBL、LNL、TIL免疫功能的比较研究  

FUNCTIONAL CHARACTERIZATION OF TUMOR-INFILTRATING LYMPHOCYTES LYMPH NODE LYMPHOCYTES AND PERIPHERAL BLOOD LYMPHOCYTES FROM PATIENTS WITH COLORECTAL CARCINOMA

作　　者：季明春[1] 龚卫娟[1] 管洪文[2] 刘歆农[2] 刘庆宏[1] 

机构地区：[1]扬州大学医学院,扬州225001 [2]扬州大学医学院附属医院,扬州225001

出　　处：《江苏临床医学杂志》2001年第4期275-279,共5页Journal of Jiangsu Clinical Medicine

基　　金：江苏省卫生厅资助项目

摘　　要：目的 :研究结肠直肠癌患者外周血淋巴细胞、肿瘤浸润淋巴细胞、肿瘤引流淋巴结淋巴细胞的免疫学功能 ,为研究结肠直肠癌患者的过继性细胞免疫治疗积累实验资料。方法 :比较结肠直肠癌患者不同来源淋巴细胞的细胞表型、NK活性、LAK活性、特异性细胞毒活性、体外增殖反应等特征。结果 :结肠直肠癌患者PBL、LNL、TIL在免疫功能上有较大差别。从结肠直肠癌患者肿瘤原位新鲜分离的LNL和TIL的NK活性、LAK活性和特异性细胞毒活性均明显低于PBL。PBL中含有较多的NK细胞 ,LNL中含有较多的B细胞和CD4+ 细胞 ,PBL和无肿瘤转移的LNL的细胞增殖能力明显高于TIL和有肿瘤转移的LNL。结论 :一般情况较好的结肠直肠癌患者外周血淋巴细胞的免疫学指标和正常人PBL相比基本正常。Objective:To study and compare the immunologic functions of tumor infiltrating lymphocytes (TIL),lymph node lymphocytes (LNL) and peripheral blood lymphocytes (PBL) in the patients with colorectal carcinoma.Methods:Tumor samples were obtained from 14 patients with colorectal carcinoma.We have compared immune biologic activity of TIL,LNL,PBL in patients with colorectal carcinoma,including the proliferous response to mitogens,phenotypic characteristics,cytotocity against tumor targets and the capability of producting IL-2.Results:The NK activity,LAK activity and specific auto-tumor cells cytotoxic activity of PBL were higher than that of TIL,LNL.Conclusions:Our findings suggest that TIL,LNL and PBL represent distinct sub-populations of lymphocytes and exhibit different functions of host immune response.

关 键 词：直肠癌 淋巴细胞 免疫特征 结肠癌 PBL LNL TIL 毒活性检测 肿瘤细胞 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]