微生物酶拆分方法生产D-泛酸的手性中间体D-泛解酸内酯  

Optical resolution of racemic DL-pantolactone by a fungal enzyme,D-lactonohydrolase

作　　者：汤一新 孙志浩[2] 华蕾[2] 过鑫富 汪军 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,无锡214036 [2]江南大学生物工程学院 [3]浙江鑫富生化股份有限公司,杭州311301

出　　处：《工业微生物》2001年第3期1-5,共5页Industrial Microbiology

摘　　要：筛选到一株产D -泛解酸内酯水解酶的串珠镰孢霉菌 (FusariummoniliformeSW -90 2 )。产酶条件研究表明 ,用甘油作碳源 ,蛋白胨作氮源 ,初始 pH8.0 ,温度 2 6℃ ,摇瓶培养 3d ,产酶量最高。在 6 0L和 10 0 0L发酵罐中通风发酵 4 5～ 4 7h ,产酶量为 6～ 8g干菌体 /L ,D -泛解酸内酯水解酶酶活力达到 0 .87～ 0 .92IU/g干菌体。该酶的最适反应温度为 5 5℃ ,最适反应 pH为 7.0～ 7.5。在酶不对称水解泛解酸内酯过程中 ,对溶液加酶量 5 %～ 10 % ,底物浓度 10 %～ 2 0 % ,控制水解率 2 0 %～ 30 % 。A strain of Fusarium moniliforme SW 902 for producing D lactonohydrolase was isolated and identified. Using glycerol as carbon source and peptone as nitrogen source, the maximum enzyme activity was obtained at 26℃ with initial pH 8.0. When the organism was cultured in the 60L or 1000L fermentor under the optimum conditions for 45～47h, 6～8g dry cells /L and 0.87～0.92 IU/g dry cell weight of specific activity were obtained. The conditions for enzyme enantioselective hydrolysis were also studied. The optimum temperature and the optimum pH for the enzyme reaction were 55℃ and pH 7.0～7.5, respectively. And with a substrate concentration of 5%～10% and enzyme concentration of 10%～20%, the maximum hydrolysis rate was obtained.

关 键 词：D-内酯水解酶 串珠镰孢霉菌SW-902 D-泛酸 手性中间体 微生物酶 拆分 D-泛解酸内酯 

分 类 号：TQ223[化学工程—有机化工]