一种新的cDNA末端快速扩增获取全长cDNA的方法  

A Novel Method of Rapid Amplification of cDNA Ends for Obtaining the Full-Length cDNA

作　　者：邱为民[1] 张思仲[1] 武辉[1] 张戈[1] 肖翠英[1] 

机构地区：[1]四川大学华西医院医学遗传研究室,成都610041

出　　处：《遗传》2001年第5期480-482,共3页Hereditas（Beijing)

基　　金：国家自然科学基金 ( 39970 40 4)资助

摘　　要：为克隆精子发生相关基因的全长cDNA ,根据mRNA差异显示获得的ESTs设计引物 ,利用一种新的cDNA末端快速扩增方法 (SMARTRACE)扩增该EST的 5′末端 ,并进行克隆测序 ,与mRNA差异显示获得ESTs拼接后 ,获得了三个新的全长cDNA。结果表明 ,SMARTRACE是一种简便、有效的克隆cDNA5′末端未知序列的技术。To clone the full-length cDNAs of genes related to spermatogenesis,ESTs obtained by mRNA differential display were used to design gene-specific primer.Then SMART RACE was performed to obtain the 5′ region of these ESTs.After cloning,sequencing and splicing with ESTs obtained by mRNA differential display,three full-length cDNAs were obtained.The results indicate that SMART RACE is a simple and an effective technique for cloning 5′-end unknown sequence of gene.

关 键 词：CDNA末端快速扩增 精子发生相关基因 睾丸 

分 类 号：Q503[生物学—生物化学]