人卵巢粘液性囊腺癌细胞系的建立及生物学特性的研究  

Establishment and characterization of a cell line derived from human ovarian mucinous cystadenocarcinoma

作　　者：万启惠[1] 徐大刚[2] 李宗权[3] 李维才[4] 吴中明[5] 程南俊[6] 

机构地区：[1]遵义医学院寄生虫学教研室,563003 [2]上海第二医科大学寄生虫学教研室 [3]成都市第六人民医院麻醉科 [4]遵义医学院电镜室,563003 [5]遵义医学院微生物学教研室,563003 [6]遵义医学院病理学教研室,563003

出　　处：《中华妇产科杂志》2001年第7期421-423,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 ( 392 60 0 74);贵州省科学基金资助项目 ( 30 84)

摘　　要：目的　探讨人卵巢粘液性囊腺癌细胞系的建立 ,并研究其生物学特性。方法　用贴壁培养法建立细胞系 ,冷冻保存。用光学显微镜、电子显微镜观察细胞系的形态 ,研究细胞系的生长及生物学特性。结果　国内第 1株人卵巢粘液性囊腺癌细胞系建立成功 ,命名为OMC685 ,现已传至 91代 ,冷冻 8年多复苏后仍生长良好。其生物学特性为 ,形态学观察证实该细胞系具有腺上皮癌细胞的特点 ;细胞生长旺盛 ;植物血凝素凝集试验阳性 ;染色体为亚三倍体 ,有畸变 ;异种动物移植阳性 ;免疫组织化学酸性粘液染色阳性 ;雌二醇及黄体酮阳性 ;无支原体及病毒污染 ;对脊髓灰质炎病毒Ⅰ型、腺病毒 7型及麻疹病毒敏感。Objective To establish a cell line of human ovarian cancer, and study its characterization. Methods The cell line was established by the cultivation of subsides walls, and kept by freezing. The morphology was observed by microscope and electromicroscope. The authors studied its growth and propagation, the agglutination test of phytohemagglutinin (PHA), the chromosome analysis, heterotransplanting, immuno histochemistry staining, the analysis of hormone, the pollution examination and the test of sensitivity to virus etc. Results A new human ovarian carcinoma cell line, designated ovarian mucinous cystadenocarcinoma 685 (OMC685), was established from mucinous cystadenocarcinoma. This cell line had subcultured to 91 generations, and some had been frozen for 8 years and revived, still grew well. This cell line possessed the feature of glandular epithelium cancer cell. The cells grew exuberantly, and the agglutinating test of PHA was positive. Karyotype was subtriploid with distortion. Heterotransplantations,alcian blue periobic acid schiff (AbPAS),mucicarmine,alcian blue stainings, estradiol (E 2) and progesterone were all positive. Without being polluted, it was sensitive to poliovirus Ⅰ, adenovirus 7 and measles virus. Conclusions OMC685 is a distinct human ovarian tumous cell line.

关 键 词：卵巢肿瘤 囊腺癌 粘液 细胞系 生物学特征 

分 类 号：R737.31[医药卫生—肿瘤] R730.21[医药卫生—临床医学]