中国人群中载脂蛋白E等位基因型与阿尔茨海默病的相关性分析  

An Association Analysis of Apolipoprotein E Genotypes with Alzheimer's Disease in Chinese Population

作　　者：吴亚宁[1] 张俊武[1] 张正馨[1] 屈秋明[1] 陈等 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《中国医学科学院学报》2001年第5期450-454,共5页Acta Academiae Medicinae Sinicae

基　　金：美国中华医学基金会资助99-699~~

摘　　要：目的研究中国人群中载脂蛋白E等位基因型与阿尔茨海默病的关联。方法利用PCR和限制性内切酶酶解,分析阿尔茨海默病患者及正常老年人载脂蛋白E各等位基因、基因型的频率。结果ApoEε2、ApoEε3、ApoEε4这三个等位基因出现的次数(和频率)在56例散发性阿尔茨海默病(AD)患者中分别为3(2.7%)、8475.0%、2522.3%,而在67例健康对照中分别为96.7%、11585.8%、107.5%。ApoEε4等位基因在AD患者中出现的频率明显高于对照组,统计学处理说明其差异有显著性(χ2=10.99P<0.01OR=3.5995%CI=1.54-8.41)ApoEε3等位基因在AD组中出现的频率低于对照组,两组中的差异也有统计学意义(P<0.05)。结论ApoEε4等位基因是AD的危险因素,ApoEε3等位基因在AD的发生中可能具有一定保护作用。Objective To analyse the association of apolipoprotein E genotypes with Alzheimer' s disease in Chinese population.Methods Using PCR and restriction enzyme digestion,we have analyzed ApoE allele frequency of Alzheimer' s disease(AD)patients and healthy controls.Results Among the56cases of AD,the frequency of ApoEε 2 ?ApoEε 3 ?ApoEε 4 are3(2.7%)?84(75.0%)?25(22.3%)respectively,while in the67controls they are9(6.7%)?115(85.8%)?10(7.5%)respectively.The frequencies of the two groups have significant differ-ence(P<0.01)and the frequency of ApoEε 4 allele is higher in AD than that in the health controls and the statistical treatment suggest that there is significant difference (χ 2 =10.99,P<0.01,OR=3.59,95%CI =1.54-8.41).The frequency of the ApoEε 3 is lower in AD than that in the health controls and the difference is also statistically significant(P<0.05).Conclusions Our results are consistent with previous work in that proving the ApoEε 4 allele is one of the risk factors of AD.The results also provide a support for the protection effect of ApoEε 3 allele in developing AD.

关 键 词：阿尔茨海默病 载脂蛋白E 等位基因型 相关性分析 

分 类 号：R749.16[医药卫生—神经病学与精神病学]