脂肪酶催化苯甘氨酸甲酯两对映体的氨解反应  

Lipase-Catalyzed Ammonolysis of Two Enantiomers of Phenylglycine Methyl Ester

作　　者：杜伟[1] 李慧青[1] 宗敏华[1] 郭勇[1] 

机构地区：[1]华南理工大学生物工程系,广东广州510640

出　　处：《分子催化》2001年第5期327-330,共4页Journal of Molecular Catalysis(China)

基　　金：广东省自然科学基金资助项目 ( No.980 5 43)

摘　　要：从 6种脂肪酶中筛选出对苯甘氨酸甲酯具有较高氨解反应活性及较高对映体选择性的脂肪酶Novozym435 ,并研究了铵盐量、温度、有机溶剂及初始水活度对两种对映体的氨解反应的影响 .当以叔丁醇为反应介质 ,反应温度为 30℃ ,铵盐量为 15 .6 mg,初始水活度为 0 .75时 。Lipase Novozym 435 was selected from six lipases due to its high activity and enantioselectivity towards ammonolysis of phenylglycine methyl ester. Effect of the amount of ammonium carbamate, temperature, solvents and initial water activity on the ammonolysis were studied. When the initial water activity is 0.75, with 15.6 mg ammonium carbamate added at 30 ℃ to 10 mL of tert\|butanol, Novozym 435 shows high ammonolysis activity and enantioselectivity.

关 键 词：脂肪酶 氨解反应 苯甘氨酸甲酯 对映体 催化反应 选择性 (R S)-苯甘氨酸 

分 类 号：O625.636[理学—有机化学] O621.251[理学—化学]