外加剂对提高固定化细胞制备L-苯丙氨酸能力的作用  

Improving the activity of immobilized cells for preparation L-Phenylalanine by additives

作　　者：周华[1] 姚忠[1] 张玮[1] 徐虹[1] 韦萍[1] 

机构地区：[1]南京化工大学制药与生命科学学院,江苏南京210009

出　　处：《南京化工大学学报》2001年第4期66-68,共3页Journal of Nanjing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金 2 0 0 760 2 1

摘　　要：在固定化细胞制备L -苯丙氨酸的研究体系中 ,以卡拉胶为固定化载体 ,所得的固定化细胞在使用过程中的催化稳定性较差 ,难以满足大规模生产的需要。故在卡拉胶包埋固定E .coli细胞过程中 ,对交联剂的处理方式和吸附剂的添加进行了研究。研究发现 ,复合交联剂对固定化细胞的处理能使固定化细胞的转化能力明显提高 :多乙烯多胺 (质量分数 0 .2 5 % )和已二胺 (质量分数 0 .2 5 % )对固定化细胞的组合处理 ,固定化细胞的转化能力提高了 3 1.6%。在细胞包埋过程中添加质量分数 4%的吸附剂E ,固定化细胞的转化能力提高了近 5 2 %。Carrageenan immobilized cells for preparation of L-Phenylalanine were stu died. In order to increase the lower catalytic stability of immobilized cell, va rious crosslinking agents and adsorbents at different methods were studied durin g the process of immobilization. It was useful for the activity of immobilized c ells raising by utilizing the combination of crosslinking agents. The activity w as increased 31.6% after immobilized cells was treated with polyethylene polyami ne (0.25%)and hexanediamide(0.25%). If adsorbent E (4%) was added during imm obilization, the activity was increased near 52%.

关 键 词：固定化细胞 L-苯丙氨酸 交联剂 吸附剂 酶催化反应 苯丙酮酸 转氨反应 卡拉胶 

分 类 号：TQ464.7[化学工程—制药化工] Q814.2[生物学—生物工程]