免疫组化法检测生殖道病变组织中的人乳头瘤病毒  

Detection of Human Papillomavirus in Histologic Lesions of Genital tract by immuno-histochemical Methods

作　　者：刘文[1] 李忠佑[1] 朱月清[1] 穆怀德 钱宏[1] 邵衡华[1] 李忠平[1] 郑国豪[2] 郑惠芳[3] 郑杰[3] 刘东风[3] 刘季和[4] 孙建方[4] 

机构地区：[1]江苏省肿瘤防治研究所,邮政编码210009 [2]安徽医科大学 [3]南京铁道医学院 [4]中国医科院皮肤病研究所

出　　处：《临床与实验病理学杂志》1991年第1期16-18,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：对宫颈炎、宫颈鳞状上皮Ⅰ—Ⅱ级不典型增生,外阴尖锐湿疣、宫颈原位癌及浸润癌共91例及男性肛门、包皮尖锐湿疣(3例)、寻常疣或扁平疣(6例)、食管癌(15例)和晚期卵巢癌(2例)用免疫过氧化物酶法(PAP法)检测组织细胞核内HPVCA。结果表明,HPVCA多见于尖锐湿疣(14/27例)、寻常疣类(4/6例)及轻中度不典型增生(6/21例)等病变组织,而且主要分布在上皮的浅表层。这些组织中也有不少挖空细胞,但两者并不完全一致。宫颈浸润癌HPVCA阳性率最低(1/17例)。卵巢癌及食管癌均呈阴性。Ninety one cases with cervicitis(6), CIN-Ⅰ-Ⅱ grade dysplasia(23), vulval condyloma acuminata(24), cervical carcinoma in situ(21) and invasive carcinoma(17), and other 26 cases with esophagealcarcinoma(15), ovarian carcinoma(2), verruca vulgaris(6) andmale anal condyloma acuminata(3) were screened for the presenceof papillomavirus antigens by immunoperoxidase (PAP) technique,using a genus specific anti-bovine papillomavirus serurn(Dakopatts).The results showed that positive PAP staining appeared in the super-ficial layer in most lesions of condyloma acuminata(14/27), verrcavulgaris(4/6) and CIN-Ⅰ-Ⅱ(6/21), and morekoilocytes in theselesions were also observed. Positivity was lowest in invasive cervicalcarcinomas(1/17) and no viral antigen werefound in esophageal andovarian carcinomas.

关 键 词：人乳头瘤病毒 免疫组化 生殖道病变 

分 类 号：R730.261[医药卫生—肿瘤]