植物基因克隆的策略和方法  

Strategies and methods for cloning plant genes

作　　者：邓洪新[1] 余懋群[1] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041

出　　处：《西南农业学报》2001年第3期78-82,共5页Southwest China Journal of Agricultural Sciences

摘　　要：植物基因克隆是当前植物学研究的前沿和热点。经过近 2 0年的发展 ,已经形成了一些克隆植物基因的方法 ,主要有功能克隆 ,PCR扩增 ,转座子或T DNA标签 ,定位克隆 ,差别杂交和减法杂交 ,mRNA差异显示和人工合成克隆。本文对这些技术的工作原理 。In the present,plant genes cloning was the front and focus in the field of botany research.After the development of about twenty years,some methods for cloning plant genes were established,which included functional cloning,PCR amplifing cloning,transposon or T DNA tagging,postional cloning,differential hybridization and subtractive hybridization,mRNA differential display and artificial synthesis DNA cloning.In this paper,these techniques theory,advantages and disadvantages of each,as well as their application prospects in cloning plant genes were introduced and dicussed.

关 键 词：植物基因克隆 功能克隆 PCR扩增克隆 转座子 T-DNA标签 MRNA差异显示 减法杂交 

分 类 号：Q943.2[生物学—植物学] S188[农业科学—农业基础科学]