盐酸间苯二胺紫外分光光度法测定亚硝酸盐的探讨与应用  

The Comment and Use of Determing Nitrite by M-Phenylenediamine Hydrochloride and Ultraviolet Fluorescence Spectrophotmoetry

作　　者：李永芳[1] 侯晓燕[1] 

机构地区：[1]安阳市卫生防疫站,河南455000

出　　处：《中国卫生检验杂志》2001年第1期17-18,共2页Chinese Journal of Health Laboratory Technology

摘　　要：基于亚硝酸盐与盐酸间苯二胺发生重氮化偶联反应，生成环偶氮化合物。建立了一种简单、快速、稳定、灵敏、准确、选择性好，测定衡量亚硝酸盐的新方法。本方法的灵敏度为０．００１μｇ／ｍｌ。测定亚硝酸盐的线性范围为０．００２－０．５μｇ／ｍｌ，能满足水和食品中痕量亚硝酸盐的测定。Based on diazo-coupling reactions between nitrite and m-phenylenediamin hydrochloride then form dzoic compound.A New method for determin trace nitrite has been studied and introduced here .This method is simple,quick and accurate and has high sensitivity and high selectivity. The sensitivity of the way is 0.001ug/ml. The linear range for determination is0. 002 - 0. 5ug/ml for nitrite. Now, it has been used to determined trace nitrite in water and food sample.

关 键 词：亚硝酸盐 紫外分光光度法 盐酸间苯二胺 水 食品 环偶氮化合物 

分 类 号：R155.51[医药卫生—营养与食品卫生学]