Huntington舞蹈病大白鼠动物模型的建立  

A MODEL OF HUNTINGTON’S DISEASE IN RAT

作　　者：李军[1] 徐慧君[1] 武义鸣[1] 冯家笙[1] 

机构地区：[1]南通医学院解剖学教研室

出　　处：《南通医学院学报》1991年第3期185-188,279+287,共4页ACTA Academiae Medicinae Nantong

摘　　要：用15μg鹅膏蕈氨酸分三点注入 SD 大鼠右侧尾壳核头部。术后1个月进行跳台试验,有29只鼠(占被破坏鼠69%)表现明显的学习记忆能力低下,且存活7个月无明显变化。光电箱试验表明,大鼠有明显的夜间过度活动。形态学观察发现,破坏后1个月鼠尾壳核头部萎缩,侧脑室扩大,破坏区神经元明显减少,胶质增生;破坏后7个月,破坏区乙酰胆碱酯酶组化染色阳性细胞及胆碱乙酰化酶、γ-氨基丁酸免疫组化染色阳性细胞明显减少,破坏侧苍白球、黑质网状部及覆盖破坏区的皮质萎缩。结果表明,该动物模型是成立的。The head of the right caudate-putamen(CP) was lesioned by three injec-tions of 5μg/0.5μl ibotenic acid(IA) in 42 rats.One month later,animalswere screened for the success of striatal denervation by a test of activeavoidance.The learning and memory behaviour was severely impaired in 29lesioned rats(69%),and was not ameliorative after seven months.In order toinvestigate the locomotor behaviour,overnight locomotor activity was meas-ured after seven months.Locomotor hyperactivity was found in the lesionedgroup.Mophologically,the rostral part of the lesioned CP was atrophiedmarkedly and the lateral ventricle was enlarged ipsilaterally,and very fewsurviving neurons were found in the lesioned area after one month.Afterseven months,AChE-histochemistry positive neurons,ChAT and GABA-immu-nohistochemistry positive neurons were decreased markedly,and even disap-peared in the lesioned area.The globus pallidus,the pars reticulata ofsubstantia nigra and the cortex overlying the lesioned CP were markedlyatrophied on the side of the lesion.The results indicate that IA lesioned model of Huntington's disease inrat is reliable.

关 键 词：舞蹈病 动物模型 鹅膏蕈氨酸 

分 类 号：R742.202[医药卫生—神经病学与精神病学]