大蒜素对人胃腺癌BGC-823细胞影响的研究  

The Study of Allicin Inducing BGC-823 Human Gastric Adenocarcinoma Cells Apoptosis

作　　者：李勇[1] 刘冀红[1] 赵群[1] 范立侨[1] 于跃明[1] 王力利[1] 赵雪峰[1] 张志栋[1] 焦志凯[1] 马志学[1] 刘品一[1] 

机构地区：[1]河北医科大学第四医院,石家庄050011

出　　处：《中国中西医结合外科杂志》2001年第5期307-310,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：河北省自然科学基金资助项目 (编号 3 993 68)

摘　　要：目的 :探讨大蒜素诱导胃癌BGC - 82 3细胞凋亡的作用及机制。 方法 :传代培养人胃腺癌BGC - 82 3细胞 ,使细胞浓度为 5× 10 5个 /ml。分别加入 10 -5g/L(大蒜素 1)和 10 -3 g/L(大蒜素 2 )的大蒜素 ,另设对照组 ,培养时间分别为 6h、2 4h、48h、72h。 结果 :与对照组相比 :①大蒜素可明显提高胃癌细胞凋亡率、降低PI值 (P <0 0 1或P <0 0 5 ) ,且表现出明显的细胞凋亡的形态学变化。②大蒜素可明显降低胃癌细胞CEA、GST -π的水平 (P <0 0 1或P <0 0 5 )。③大蒜素可明显提高胃癌细胞的cAMP水平 (P <0 0 1) ,降低PKC水平 (P <0 0 1或P <0 0 5 )。④大蒜素能够显著增加胃癌细胞fas基因及bax基因蛋白的表达 (P <0 0 5或P <0 0 1) ;降低bcl- 2基因蛋白的表达 (P <0 0 1)。 结论 :大蒜素可通过对第二信号系统cAMP、PKC的影响 ,增强细胞凋亡始动基因fas、凋亡促进基因bax的表达 ,抑制凋亡抑制基因bcl- 2的表达 ,增加细胞凋亡率 ,并使胃癌细胞调亡 ,并抑制其增殖活性 ;并可导致胃癌细胞CEA、GST -π的合成减少 ,从而降低其水平。To investigate the effect of allicin on cultured human gastric adenocancer cell line BGC-823 from the morphologic changes,proliferation,production of tumor markers,the second signal system,cell period ,expression of apoptotic gene by morphological observation,colorimetry,radioimmunoassay and flow cytometry Methods:Cultured human gastric adenocancer cells BGC-823(5×10 5/ml)were incubated in different concentrations of allicin(10 -5 g/L,10 -3 g/L),to be compared with a control group.In each group the experiment was repeated for 6 times.Results:Compared with the experimental control group,(1)In the allicin groups the apoptotic rate was significantly increased apoptotic morphosis clearly shown.(2)in the allicin groups the contents of CEA,GST π were significantly decreased.(3)cAMP was significantly increased and PKC was significantly reduced in cells in the allicin groups.(4)The expression of fas and bax genes increased and that of bcl-2 gene reduced.Conclusion:Allicin induced BGC-823 cell apoptosis by affecting cAMP,PKC and increasing the expression of fas and bax genes and inhibiting that of bcl-2 gene.Allicin was proved to reduce malignancy of gastric cancer by decreasing the production of tumor markers.This study will support the clinical use of allicin to gastric cancer,and combined chemotherapy with experimental basis.

关 键 词：大蒜素 胃腺癌 BGC-823细胞 

分 类 号：R735.2[医药卫生—肿瘤]