血小板源生长因子β受体反义寡核苷酸对血小板源生长因子诱导大鼠血管平滑肌细胞迁移的影响  

Effect of Platelet Derived Growth Factor β Receptor Antisense Oligonucleotide on Platelet Derived Growth Factor Induced Migration of Rat Vascular Smooth Muscle Cells

作　　者：顾春虎[1] 侯英萍[1] 李焰[1] 乔宏庆[1] 

机构地区：[1]第四军医大学西京医院核医学科,西安710032

出　　处：《中国动脉硬化杂志》2001年第5期394-397,共4页Chinese Journal of Arteriosclerosis

基　　金：第四军医大学创新工程项目 (CX99A0 0 6 )资助

摘　　要：为研究血小板源生长因子 β受体反义寡核苷酸对血小板源生长因子诱导的大鼠血管平滑肌细胞迁移的影响和作用机制 ,利用激光共聚焦显微镜观察平滑肌细胞对异硫氰酸荧光素标记的反义寡核苷酸的摄取和细胞内定位 ,改良Boyden’s小室法观察平滑肌细胞迁移。结果发现 ,加入反义寡核苷酸培养 2 4h及 48h后荧光分别位于细胞浆内和细胞核内 ;5 μmol L以上浓度反义寡核苷酸作用 36h后 ,被血小板源生长因子诱导迁移通过Boyden’s小室碳纤维素膜的细胞数量少于空白对照组 ;正义、错义寡核苷酸和 5 μmol L以下浓度反义寡核苷酸迁移抑制不明显。提示血小板源生长因子β受体反义寡核苷酸在细胞核内呈时间和浓度依赖性抑制血小板源生长因子诱导的血管平滑肌细胞迁移。Aim To investigate the effect of platelet derived growth factor β-receptor (PDGFR-β)antisense oligonucleotides (AODN) on platelet derived growth factor (PDGF)induced migration of cultured rat vascular smooth muscle cells(VSMC).Methods In vitro cultured rat VSMC model was established. Interactive Laser Cytometer (ILC) was used to investigate fluoresceinisothiocyanate labeled PDGFR-β AODN cellular uptake and localization. The migration of VSMC was assayed by a modification of Boyden's chamber method. Results Fluorescent AODN localized in the cytoplasm of VSMC at 24 hours and in the nucleus at 48 hours. Compared with control group, PDGFR-β AODN at dose of 5 μmol/L or higher can significantly decrease the number of cells which had migrated through the polycarbon filter induced by PDGF. PDGFR-β AODN at dose of 2.5 μmol/L or lower, sense oligonucleotide and scrambled oligonucleotide could not significantly inhibit PDGF induced migration of VSMC. Conclusion PDGFR-β AODN can localize in the nucleus and significantly inhibit PDGF induced migration of cultured VSMC in a dose and time-dependent fashion.

关 键 词：反义寡核苷酸 再狭窄 血小板源生长因子 血管平滑肌 

分 类 号：R363[医药卫生—病理学]