嗜碱芽孢杆菌XE22-4-1碱性弹性蛋白酶发酵条件的研究  

FERMENTATION CONDITIONS FOR PRODUCTION OF ALKALINE ELASTASE BY ALKALIPHILIC BACILLUS XE22-4-1

作　　者：肖昌松[1] 吕健[1] 田新玉[1] 李向前[1] 周培瑾[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2001年第5期611-616,共6页Acta Microbiologica Sinica

基　　金：国家科技部"九五"攻关项目 ( 96 C0 3 0 2 0 4 )&&

摘　　要：从西藏天然碱湖中筛选到一株产碱性弹性蛋白酶 (alkalineelastase)菌株 ,最适生长pH为 1 0 0 ,经鉴定为Bacillussp .,编号XE2 2 4 1。该菌产酶最适碳源为 2 %葡萄糖 ,氮源为0 2 5%酵母粉 ,豆饼粉对发酵产酶有促进作用。 2L发酵罐实验表明 ,溶解氧是影响该菌株产酶的重要因素。通过提高通气量和改变搅拌速度 ,该菌株可在发酵 48h内达到产酶高峰 ,酶活力最高达 2 66uA extracellular alkaline elastase producing bacterial strain Bacillus XE22\|4\|1, with the optimum pH of 10.0 for its growth, was isolated and screened from alkali lake of Tibet. 2% glucose and 0.25% yeast extract are respectively the appropriate carbon and nitrogen source on elastase production. Soymeal can promote elastase production. The fermentation conditions in a 2 L stirred tank were investigated. the results have revealed that the dissolved oxygen is the most effective factor on elastase production. The maximum elastolytic activity reached 266 u/mL among 48 hours of fermentation by combination of enhancing aeration and changing the mixing speed.

关 键 词：碱性弹性蛋白酶 嗜碱芽孢杆菌 发酵条件 

分 类 号：TQ920[轻工技术与工程—发酵工程]