粗山羊草高分子量麦谷蛋白新型亚基的筛选和鉴定  

Identification of Novel High-molecular-weight Glutenin Subunits from Aegilops Tauschii

作　　者：颜泽洪[1] 郑有良[1] 万永芳[2] 刘坤凡[2] 王道文[2] 

机构地区：[1]四川农业大学小麦研究所,四川都江堰611830 [2]中国科学院遗传研究所,北京100101

出　　处：《四川农业大学学报》2001年第3期197-199,217,共4页Journal of Sichuan Agricultural University

基　　金：四川省科技厅科研项目 ;四川省教育厅科研项目

摘　　要：利用SDS -PAGE和Western Blotting分析与鉴定技术 ,从 5 1份粗山羊草中共发现了 2种新型亚基Tx >2和Ty <12 ,被命名为Dx2 1t 和Dy13t;7种亚基组合类型 2 +12、5 +10、2 +10、5 +12、2 1t+10、2 1t+12和 2 +13t,这7种亚基组合的频率分别是 2 7 5 %、2 5 5 %、31 4%、3 9%、2 0 %、2 0 %和 7 8%。已将新型亚基Dx2 1tThe high molecular weight glutenin subunits compositions of 51 accessions of Aegilops tauschii were investigated by using SDS-PAGE analysis and Western blotting technical. Two novel types of high molecular weight glutenin subunits, Tx>2 (with mobility faster than the Dx2 and Dx5 of common wheat) and Ty<12 (with mobility slower than the Dy10 and Dy12 of common wheat) were observed, which were designated as Dx2 1 t and Dy13 t, respectively. The frequencies of 7 subunit combinations (2+12, 5+10, 2+10, 5+12, 2 1 t+10, 2 1 t+12 and 2+13 t) were 27 5%, 25 5%, 31 4%, 3 9%, 2 0%, 2 0%和7 8%, respectively. We have cloned the novel high molecular glutenin subunits Dx2 1 t and Dy13 t.

关 键 词：粗山羊草 小麦 高分子量麦谷蛋白亚基 亚基组合 杂交种 

分 类 号：S512.1[农业科学—作物学]