肺癌基因疫苗抑制肿瘤的实验研究  

Experimental study of lung carcinoma gene vaccine for inhibiting effect of mouse tumor

作　　者：胡伟恩[1] 刘祥麟[2] 于晓虹[2] 

机构地区：[1]杭州市第一人民医院,浙江310006 [2]浙江大学医学院,杭州310006

出　　处：《临床肿瘤学杂志》2001年第4期309-310,313,共3页Chinese Clinical Oncology

基　　金：杭州市科委科研基金资助(编号99113A07)

摘　　要：目的:研究肺癌基因疫苗对小鼠免疫原性及对荷瘤动物的治疗作用。方法:利用逆转录病毒载体ＰＬＸＳＮ将ｒ－ＩＦＮ基因导入人肺癌 Ａ５４９细胞中,３６只小鼠分６组,每只小鼠给予皮下注射０．５×１０４/０．２ｍｌ Ｓ１８０细胞分为实验各组和对照组。结果:基因疫苗组抑瘤率为８３．３%,对照组抑瘤率为１６．６%。结论:初步研究提示肺癌疫苗对肿瘤有明显抑制作用。ve: To investigate the effect of transgenic human lung adenocarcinoma cell line A549/IFN - r as tumor vaccine on immunogenicity and inhibition of animal tumor. Method: Interferon - gamma(IFN - r)gene was introduced into human lung adenocarcinoma cell line A549 and was expressed stably on the basis of the constraction of retroviral packing cell line PA317/PLXSN. Total 36 mice into six groups, each mouse was inoculated 0.5 × 104 /0.2ml S180 cells in subcutaneous.Results: The result indicated that the 83.3 percent has obtained tumor inhibition from the gene vaccine group, and control group is 16.7% only. Conclusion: The initial experiment suggested that the transgenic human lung adenocarcimoma cell vaccine A549/IFN - r is possessed of suppression effect for mouse tumor.

关 键 词：肺癌 基因疫苗 免疫疗法 

分 类 号：R734.2[医药卫生—肿瘤]