嗜碱细菌复合碳源条件下对麦草木质素的降解  

Biodegradation of Lignin in Wheat Straw by Alkaliphilic Ligninolytic Bacteria with Compounded Carbons

作　　者：张甲耀[1] 龚利萍[1] 罗宇煊[1] 许威亚[2] 凌颉[2] 

机构地区：[1]武汉大学环境科学系,武汉430072 [2]武汉大学分析测试中心

出　　处：《环境科学》2002年第1期70-73,共4页Environmental Science

基　　金：国家自然科学基金资助课题 (2 96 770 12 )

摘　　要：在碱性液体培养条件下 ( pH≈ 1 0 .5 ) ,研究复合碳源共代谢最佳综合条件下嗜碱性木质素降解细菌 6号菌株产酶、降解能力及菌株的生长状况 .结果显示 ,虫漆酶 (Laccase)在培养的第 4天酶活达到最高值 2 91 5 37U/L、锰依赖过氧化物酶 (MnP)在培养的第 8天酶活达到最高值 1 1 5 2 88U/L ,培养 1 0d麦草中木质素降解 4 9 84 % .同时通过扫描电镜分析探讨了 6号菌株降解木质素的微观过程 ,证明了The article researched enzymes production、the degradation rate of lignin and strain growth of alkaliphilic ligninolytic bacteria strain No.6 in alkaline liquid medium (pH≈10.5) with compounded carbons. The results showed that the highest activities of laccase and MnP were 2915 37U/L(4th d) and 1152 88U/L(8 th d), respectively, and the strain degraded 49 84% lignin of wheat straw during ten days cultivation. The changes of ultrastructure of wheat straw caused by strain No.6 attack were investigated with SEM (scan electron microscopy).

关 键 词：嗜酸细菌 复合碳源 木质素 生物降解 扫描电镜 麦草 微生物降解 造纸 废水处理 

分 类 号：X172[环境科学与工程—环境科学] X793