神经母细胞瘤增殖、凋亡与休眠消退的相关性研究  

Study on the Correlation between the Proliferation or Apoptosis of and the Dormancy or Regression of Neuroblastoma

作　　者：毕红梅 何延兴 毕迅[2] 

机构地区：[1]上街区人民医院,河南郑州450041 [2]首都儿科研究所,北京100020

出　　处：《河南肿瘤学杂志》2001年第6期397-398,共2页Henan Journal of Oncology

摘　　要：目的　本实验通过研究神经母细胞瘤的增殖、凋亡的改变 ,结合神经母细胞瘤的分化 ,以探讨增殖、凋亡的改变与神经母细胞瘤休眠消退的关系。方法　肿瘤休眠消退组 :神经母细胞瘤休眠消退 6例 ,男 4例 ,女 2例。取组织制作成石蜡切片 ,运用免疫组织化学方法检测增殖细胞 ,计算增殖细胞占细胞百分比 ,求得增殖指数 (PI)。运用原位DNA末端标记法 ,检测凋亡细胞 ,计算凋亡细胞占细胞百分比 ,求得凋亡指数 (AI)。结果　休眠消退病例中 5例凋亡指数大于 9% ,5例增殖指数大于 13 % ,6例增殖率减凋亡率均小于 4%。结论　增殖指数小于 13 % ,凋亡指数大于 9% ,特别是增殖指数减凋亡指数小于 4% ,神经母细胞瘤休眠消退的可能性大。Objective To explore the relation between the proliferation or apoptosis and the dormancy or regression of human neuroblastoma.Methods In the dormancy or regression tumor group, there were 6 neuroblastoma, including 4 male and 2 female, PCNA was identified immunohistochemically and the percentage of the PCNA-positive unclei(PCNA index:PI) was calculated, Apoptosis was determined by in situ endlabeling of fragmented DNA and the percentage of apoptosis-positive nuclei(apoptosis index:AI) was also calculated.Results PI in the 5/6 patients was less than 13%, while AI in other 5/6 patients was greater than 9%.In addition, PI minus AI was less than 4% in all of the 6 patients.Conclusions It is possible to have the dormancy or the regression of neuroblastoma when PI is less than 13% or AI is greater than 9%, what is more when, PI minus AI is less than 4%.

关 键 词：神经母细胞瘤 增殖 休眠 凋亡 消退 

分 类 号：R737.11[医药卫生—肿瘤]