毛细管电泳电化学检测法测定红葡萄酒中的多元酚  

Determination of Phenolic Compounds in Red Wine by Capillary Zone Electrophoresis with Electrochemical Detection

作　　者：曹玉华[1] 张欣[1] 方禹之[1] 叶建农[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《高等学校化学学报》2001年第12期2011-2013,共3页Chemical Journal of Chinese Universities

基　　金：中国科学院长春应用化学研究所电分析化学实验室资助

摘　　要：A simultaneous determination of (-) epictechin, (+) catechin, gentisic acid, ferulic acid, quercetin and gallic acid in red wine by capillary zone electrophoresis with electrochemical detection(CE ED) was reported. The effects of potential of working electrode, pH and concentration of running buffer, separation voltage and injection time on CE ED were investigated. Under the optimum conditions, these six analytes could be separated in a 100 mmol/L borate buffer(pH=9 24) within 30 min. A 300 μm diameter carbon disk electrode has a good response at +0 85 V( vs . SCE) for all analytes. The responses were linear over two orders of magnitude with detection limit( S/N =3) ranged from 7×10 -8 g/mL to 1 4×10 -7 g/mL for all six analytes. This method has been used for the determination of these analytes in red wine without enrichment, and the assay result was satisfactory.A simultaneous determination of (-) epictechin, (+) catechin, gentisic acid, ferulic acid, quercetin and gallic acid in red wine by capillary zone electrophoresis with electrochemical detection(CE ED) was reported. The effects of potential of working electrode, pH and concentration of running buffer, separation voltage and injection time on CE ED were investigated. Under the optimum conditions, these six analytes could be separated in a 100 mmol/L borate buffer(pH=9 24) within 30 min. A 300 μm diameter carbon disk electrode has a good response at +0 85 V( vs . SCE) for all analytes. The responses were linear over two orders of magnitude with detection limit( S/N =3) ranged from 7×10 -8 g/mL to 1 4×10 -7 g/mL for all six analytes. This method has been used for the determination of these analytes in red wine without enrichment, and the assay result was satisfactory.

关 键 词：毛细管电泳 电化学检测 红葡萄酒 多元酚 分析 

分 类 号：TS262.6[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]