散发性肌萎缩侧索硬化病人脑脊液中3-硝基酪氨酸水平增高  

The increase of 3-nitrotyrosine in cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis

作　　者：鲁明[1] 樊东升[1] 康德瑄[1] 耿秋明[2] 陈清棠[3] 

机构地区：[1]北京大学第三医院神经科,100083 [2]北京大学第三医院中心实验室,100083 [3]北京大学第一医院神经科

出　　处：《中华神经科杂志》2001年第6期351-354,共4页Chinese Journal of Neurology

基　　金：教育部留学回国人员科研启动基金 ;安万特医药ALS研究基金资助项目

摘　　要：目的　探讨自由基氧化损伤在散发性肌萎缩侧索硬化的发病机制中有无作用。　方法散发性肌萎缩侧索硬化病人 15例 ,神经系统正常的外科手术腰麻病人 2 0例 ,采集脑脊液后用高效液相色谱法进行检测分析。比较散发性肌萎缩侧索硬化病人和神经系统正常的受试者脑脊液中 3 硝基酪氨酸、酪氨酸含量及 3 硝基酪氨酸与酪氨酸比值。结果　散发性肌萎缩侧索硬化病人脑脊液中 3 硝基酪氨酸含量及 3 硝基酪氨酸与酪氨酸比值与对照组相比增高有显著性差异 :病人组与对照组脑脊液中 3 硝基酪氨酸含量分别为 (2 2 8 5 2± 12 4 30 )nmol L和 (112 86± 47 10 )nmol L ,P <0 0 1;两组的 3 硝基酪氨酸与酪氨酸比值分别为 (7 6 0± 4 38)× 10 3 和 (3 19± 1 2 0 )× 10 3 ,P <0 0 1。结论　由过硝酸根介导的自由基氧化损伤在散发性肌萎缩侧索硬化的发病机制中具有重要作用。Objective To compare the concentration of 3-nitrotyrosine and tyrosine and the ratio of 3-nitrotyrosine/tyrosine in the cerebrospinal fluid (CSF) of patients with sporadic amyotrophic lateral sclerosis (SALS) and those with healthy nervous system for definiting the role of free radicals oxidative damage in pathogenesis of SALS. Methods Subjects consisted of 15 patients with SALS and 15 patients with norml nervous system who underwent lumbar anesthesia for surgery. Their CSF was analyzed by using high performance liquid chromatography (HPLC). Results The 3-nitrotyrosine concentration was higher in patients with SALS (228.52±124.30 nmol/L) than that in controls (112.86±47.10 nmol/L) (P<0.01). The 3-nitrotyrosine/tyrosine ratio was significantly higher in patients with SALS [(7.60±4.38) ×10 -3] than that in controls [(3.19±1.20) ×10 -3] (P<0.01). Conclusion Free radicals oxidative damage mediated by peroxynitrite plays an important role in the SALS pathogenesis .

关 键 词：肌萎缩侧索硬化 酪氨酸 脑脊液 

分 类 号：R744.8[医药卫生—神经病学与精神病学]