转Bt基因棉花及其受体品种主要挥发性物质的测定  

Analysis of volatile components in transgenic Bt cotton and their parental varieties

作　　者：张永军[1] 徐广[1] 郭予元[1] 吴孔明[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100094

出　　处：《生态学报》2001年第12期2051-2056,共6页Acta Ecologica Sinica

基　　金：国家自然科学基金 ( No.39770 4 98);国家重点基础研究发展规划项目 ( No.G2 0 0 0 0 1 6 2 0 8)资助项目

摘　　要：应用顶空进样气质联机系统 ( Headspace-GC-MS)测定了转 Bt基因棉花及其对照亲本主要挥发性物质。结果表明棉花营养器官和繁殖器官的主要挥发性物质有差异 ,棉花植株现蕾期前 ,叶片中挥发性物质以 α-蒎烯为主 ,而现蕾后 ,蕾、花和铃中主要以β-月桂烯为主。研究初步发现 ,外源By using the headspace collection and GC-MS, the differences in composition and concentrations of volatile allelochemics between transgenic Bt cotton and their parental varieties during various developmental stages were compared and analyzed. The cotton varieties DP5415-NUCOTN33 B, Simian3-GK2 and CCRI16-CCRI30 of both the parental variety and Bt transgenic cotton, were planted in an experimental field of Institute of Plant Protection, CAAS. The following plant parts in cotyledon-, three-leaf-, seven-leaf-, squaring-, flowering- and boll-stage, respectively, were used for volatile collections: cotyledon, top tender leaf, small square, floral organ, small boll. The samples were analyzed with GC-MS. HP6890 GC equipped with HP 5MS capillary column and HP5973 MS were used for chemical analysis. The carried gas was helium. The temperature program was as following: 40℃was maintained for 1 min and then the temperature was increased at 20℃/min to 100℃ and maintained for 10 min. The temperature at both the entrance and detector was 250℃. Current velocity of carried gas in GC was 50 ml/min and the split ratio was 28:1. The current velocity in column was 1.6ml/min. The source temperature of MS was 260℃. The main volatile compounds in cotton organs collected during various developmental stages were detectable and well separated with GC. The following compounds were identified: in cotton leaf— α-pinene, camphene, cyclohexene, β-pinene, β-myrcene, 1,3-pentadiene-3-methyl, β-hellandrene, D-limonene, 3-carene and a trace of hexen-2-ol-1; in cotton square and flower — α-pinene, β-pinene, β-phellandrene; in addition to the similar volatile allelochemics contained in square and flower, the cotton boll contained 3-carene as well. The dominant compound in cotton leaf was α-pinene (60%), and that in square, flower and boll was β-myrcene (80%). The results indicated that the components and ratios of main volatile allelochemics in various parts of cotton are different, thus it can be inferred that the chemical

关 键 词：转BT基因棉花 挥发性物质 气质联机系统 测定 亲本品种 

分 类 号：S435.622.9[农业科学—农业昆虫与害虫防治]