PELA疫苗微球载体的正交优化工艺研究  

TECHNIC STUDY ON THE PELA MICROSPHERE VEHICLE LOADED VACCINE BY ORTHOGONAL EXPERIMENTS

作　　者：任建敏[1] 邹全明[1] 彭承琳[2] 田文标[1] 张玮[1] 

机构地区：[1]第三军医大学检验系,重庆400038 [2]重庆大学生物工程学院,重庆400044

出　　处：《华西药学杂志》2002年第1期7-9,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的　利用正交设计的数学方法进行实验设计 ,以牛白蛋白为包裹模型 ,寻求制备不同疫苗微球载体的最佳条件。方法　采用W /O/W法制备PELA微球 ,考察不同因素对疫苗微球平均粒径、成球率与蛋白包裹率 (三指标 )的影响。结果　五因素对疫苗微球成球性影响的主次顺序为PVA浓度 >搅拌速度 >PELA浓度 >油外 /外水相体积比 >内水相体积 ;而对粒径与蛋白包裹率的顺序则为PELA浓度 >PVA浓度 >搅拌速度 >油相 /外水相体积比 >内水相体积和PELA浓度 >PVA浓度 >搅拌速度 >内水相体积 >油相 /外水相体积比。结论　控制工艺条件 。OBJECTIVE To Explore the optimization conditions to prepare the microsphere vehicle loaded BSA.METHODS Microsphere vehicle loaded BSA were prepared by double emulsion method(W/O/W)with orthogonal experiments.RESULTS The sequences of the five factors affecting their properties are below.Shaping sphere efficiency:Concentration of PVA→velocity of stirring→concentration of polymer→O/W outer water phase →volume of the inter water phase;Mean diameter:Concentration of polymer →concentrationof PVA →velocity of stirring →O/W outer water phase →volume of the inter water phase;Protein encapsulation efficiency:Concentration of polymer →concentration of PVA →velocity of stirring →volume of the inter water phase →O/W outer water phase CONCLUSION The microsphere vehicle loaded vaccine for some demands can be prepared by controlling its technic conditions.

关 键 词：正交优化 疫苗微球 牛白蛋白 PELA疫苗 微球载体 

分 类 号：R914[医药卫生—药物化学]